Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Anammox Bacteria Enrichment in Upflow Anaerobic Sludge Blanket (UASB) Reactor

  • Thuan Tran-Hung (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Jahng Deok-Jin (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Jung Jin-Young (Environment and Process Technology Division, Korea Institute of Science and Technology (KIST)) ;
  • Kim Dong-Jin (Department of Environmental Systems Engineering, Hallym University) ;
  • Kim Won-Kyoung (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Park Young-Joo (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Kim Ji-Eun (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Ahn Dae-Hee (Department of Environmental Engineering and Biotechnology, Myongji University)
  • Published : 2004.10.01
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Abstract

We investigated the anaerobic ammonium oxidation (anammox) reaction in a lab-stale upflow anaerobic sludge blanket (UASB) reactor. Our aim was to detect and enrich the organisms responsible for the anammox reaction using a synthetic medium that contained low concentrations of substrates (ammonium and nitrite). The reactor was inoculated with granular sludge collected from a full-scale anaerobic digestor used for treating brewery wastewater The experiment was performed during 260 days under conditions of constant ammonium concentration ($50\;mg\;NH_4^+-N/L$) and different nitrite concentrations ($50{\~}150\;mg\;NO_2-N/L$). After 200 days, anammox activity was observed in the system. The microorganisms involved in this anammox reaction were identified as Candidatus B. Anammoxidans and K. Stuttgartiensis using fluorescence in situ hybridization (FISH ) method.

Keywords

References

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