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The Korean Association of Immunobiologists (대한면역학회)
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Enhancement of Nitric Oxide Production by Corticotropin-releasing Hormone (CRH) in Murine Microglial Cells, BV2

생쥐 미세아교세포(BV2)에서 Corticotropin-releasing Hormone (CRH)에 의한 Nitric Oxide (NO) 생성의 증가

  • Yang, Yool-hee (Department of Life Science, Sookmyung Women's University, Laboratory of Immunology) ;
  • Yang, Young (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Dae-Ho (Department of Life Science, Sookmyung Women's University, Laboratory of Immunology)
  • 양율희 (숙명여자대학교 이과대학 생명과학과 면역학실험실) ;
  • 양영 (한국 생명과학연구소 면역학실험실) ;
  • 조대호 (숙명여자대학교 이과대학 생명과학과 면역학실험실)
  • Published : 2004.03.31
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Abstract

Background: Microglial cells, major immune effector cells in the central nervous system, become activated in neurodegenerative disorders. Activated microglial cells produce proinflammatory mediators such as nitric oxide (NO), tumor necrosis factor-$\alpha$ and interleukin-$1{\beta}$(IL-$1{\beta}$). These proinflammatory mediators have been shown to be significantly increased in the neurodegenerative disorders such as Alzhimer's disease and Pakinson's disease. It was known that one of the neurodegeneration source is stress and it is important to elucidate mechanisms of the stress response for understanding the stress-related disorders and developing improved treatments. Because one of the neuropeptide which plays a main role in regulating the stress response is corticotropin-releasing hormone (CRH), we analyzed the regulation of NO release by CRH in BV2 murine microglial cell as macrophage in the brain. Methods: First, we tested the CRH receptor expression in the mRNA levels by RT-PCR. To test the regulation of NO release by CRH, cells were treated with CRH and then NO release was measured by Griess reagent assay. Results: Our study demonstrated that CRH receptor 1 was expressed in BV2 murine microglial cells and CRH treatment enhanced NO production. Furthermore, additive effects of lipopolysaccaride (LPS) and CRH were confirmed in NO production time dependantly. Conclusion: Taken together, these data indicated that CRH is an important mediator to regulate NO release on microglial cells in the brain during stress.

Keywords

Acknowledgement

Supported by : 한국 학술진흥 재단

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