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The Korean Association of Immunobiologists (대한면역학회)
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Effect of $\beta_2$-GP1 on the Binding of Anti-cardiolipin Antibodies to Cardiolipin

Anti-cardiolipin 항체와 Cardiolipin의 결합에 미치는 $\beta_2$-GP1의 영향

  • Kang, Eun-Young (Laboratory of Immunology, Institute for Medical Sciences, Ajou University Schools of Medicine) ;
  • Jang, Young-Ju (Laboratory of Immunology, Institute for Medical Sciences, Ajou University Schools of Medicine)
  • 강은영 (아주대학교 의과대학 의과학연구소 면역학연구실) ;
  • 장영주 (아주대학교 의과대학 의과학연구소 면역학연구실)
  • Published : 2004.09.30
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Abstract

Background: Anti-cardiolipin antibody (Anti-CL Ab) is one of the various antiphospholipid antibodies (Anti-PL Abs) and found in the plasma of patients with systemic lupus erythematosus (SLE), atherosclerosis, and other infectious diseases. While anti-PL Abs found in the sera of patients with infectious diseases bind directly to CL, binding of anti-PL Abs to CL circulating in the sera of patients with autoimmune diseases is mediated by $\beta_2-$glycoprotein 1 ($\beta_2-GP1$). The purpose of this study is to investigate the effect of <$\beta_2-GP1$ on the antigen binding assay of anti-CL Abs present in the sera of patients with atherosclerosis, which has been known as one of autoimmune diseases. Methods: ELISA was performed with sera containing anti-CL Abs from three patients with atherosclerosis in the presence or absence of $\beta_2-GP1$ or FBS. Results: Reactivity of anti-CL Abs to CL was increased in the presence of $\beta_2-GP1$ or FBS in a dose dependent manner. Conclusion: <$\beta_2-GP1$ or FBS could be used as co-factor in CL ELISA with anti-CL Abs present in the sera of patients with atherosclerosis. It is suggested that anti-CL Abs found in atherosclerosis patients are similar in terms of antigen binding property to those circulating in the patients with autoimmune diseases, not to infectious diseases.

Keywords

References

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