Asian-Australasian Journal of Animal Sciences

  • 제17권1호
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  • Pages.33-38
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  • 2004
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Expression of the Antioxidant Enzyme and Apoptosis Genes in In vitro Maturation/In vitro Fertilization Porcine Embryos

  • Jang, H.Y. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Lee, S.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Choi, K.D. (East West Kidney Disease Research Institute, School of Medicine, Kyung Hee University) ;
  • Jeon, G.J. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Yang, B.K. (Division of Animal Resources Science, Kangwon National University) ;
  • Lee, C.K. (School of Agricultural Biotechnology, Xenotransplantation Research Center, Seoul National University) ;
  • Lee, H.K (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
  • 투고 : 2003.05.14
  • 심사 : 2003.09.06
  • 발행 : 2004.01.01
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초록

This study was aimed at testing the gene expression of antioxidant enzymes and apoptosis genes for in vitro culture in porcine embryos produced by in vitro maturation/in vitro fertilization (IVM/IVF). Pocine preimplantation embryos obtainted from IVM/IVF can be successfully culture in vitro, but they are delayed or stop to develop at specific developmental stage. Many factors such as reactive oxygen species and apoptosis in an IVM/IVF system followed by in vitro culture influence the rate of production of viable blastocysts. Porcine embryos derived from IVM/IVF were cultured in the atmosphere of 5% $CO_2$ and 20% $O_2$ at $38.5^{\circ}C$ in NCSU23 medium. The patterns of gene expression for antioxidant enzymes and apoptosis genes during in vitro culture in pocine IVM/IVF embryos were examined by the modified semi-quantitative single cell reverse transcriptase-polymerase chain reaction (RT-PCR). Porcine embryos produced by in vitro procedures were expressed mRNAs for CuZn-SOD, GAPDH and GPX, whereas transcripts for Mn-SOD and catalase were not detected at any developmental stages. Expression of caspase-3 mRNA was detected at 2 cell, 8 cell 16 cell and blastocyst, but p53 mRNA was not detected at any stages. The fas transcripts was only detected in blastocyst stage. These results suggest that various antioxidant enzymes and apoptosis genes play crucial roles in vitro culture of porcine IVM/IVF embryos.

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