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Antioxidant Enzymes in Postpartum Anoestrus Buffaloes Supplemented with Vitamin E and Selenium

  • Anita, Anita (Dept. of Animal Biochemistry & F.S.I.V.R.I., Izatnagar) ;
  • Singha, S.P.S. (Department of Veterinary Biochemistry Punjab Agricultural University) ;
  • Dhillon, K.S. (Department of Soil Science, Punjab Agricultural University) ;
  • Nayyar, Shashi (Department of Veterinary Anatomy and histology, Punjab Agricultural University)
  • Received : 2002.09.17
  • Accepted : 2003.04.04
  • Published : 2004.05.01

Abstract

The 15 buffaloes were divided into three groups, viz. group 1: normal cyclic buffaloes; group 2: postpartum anoestrus buffaloes and group 3: post partum anoestrus buffaloes supplemented with intramuscular injections of Vit. E.-care Se containing 500 mg $\alpha$-tocopheryl acetate and 15 mg selenium at weekly intervals for two months. The postpartum anoestrus buffaloes had significantly higher levels of erythrocytic lipid peroxidation, superoxide dismutase and glucose-6 phosphate dehydrogenase activities but lower glutathione peroxidase activity as compared to normal cyclic buffaloes. The supplementation of vitamin E and selenium lowered the level of erythrocytic lipid peroxidation, superoxide dismutase and glucose-6 phosphate dehydrogenase activities but it had no effect on whole blood selenium and erythrocytic gluathione peroxidase activity. All the animals in group 3 became cyclic and showed 60% conception rate.

Keywords

References

  1. Bieleski, H. K., P. Weber and H. E. Weiss. 1997. Antioxidative vitamins in prevention. Vitamin: Physiologie Patho Physiology Theraphie: 206-228.
  2. Brezezinska, S. E., J. Miller, I. D. III Quigley, J. R. Moore and F. C. Madsen. 1994. Antioxidant status of dairy cows supplemented prepartum with vitamin E and selenium. J. Dairy Science 77:3087-3095.
  3. Deutsch, J. 1978. Maleimide as an inhibitor in measurement of erythrocyte glucose-6-phosphae dehydrogenase activity. Clin. Chem. 24:885-889.
  4. Hafeman, D. G., R. A. Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr. 104:580-587.
  5. Kim, H. S., J. M. Lee, S. B. Park, S. G. Jeag, J. K. Jung and K. S. Im. 1997. Effect of vitamin E and selenium administration on the reproductive performance in the dairy cows. Asian-Aust. J. Anim.Sci. 10:308-312.
  6. Koh, T. S. and T. H. Benson. 1983. Critical reappraisal of fluorometric method for determination of selenium in biological materials. Assoc. Off. Anal. Chem. J. 66:918-925.
  7. Nishikimi, M., N. A. Rao. and K. Yasgi. 1972. The occurrence of the superoxide anion in the reaction of reduced phenazine methsulfate and molecular oxygen. Biochem. Biophys. Res. Commun. 46:849-854.
  8. Pironi, L., E. Ruggeri, C. Zolezzi and L. Sarvarino.1998. Lipid peroxidation and antioxidant status in adult receiving lipid based home parental nutrition. Am. J. Clin. Nutr. 68:888-893.
  9. Placer, Z. A., L. L. Cushman and B. C. Johnson. 1966. Estimation of product of lipid peroxidation (Malonyl dialdehyde) in biochemical systems. Anal. Biochem. 16:359-364.
  10. Singh, S., T. P. Singh, M. L. Bansal and R. Kumar. 1991. Statistical Methods for Research Workers. pp.310-312. Kalyani Publisher, New Delhi.
  11. Walsh, D. M., S. Kennedy, W. J. Blanchflower, E. A. Goodall and D. G. Kennedy. 1993a. Vitamin E and selenium deficiencies increase indices of lipid peroxidation in rumiant calves. J. vit. Nutr.Res. 63:188-194.
  12. Walsh, D. M., D. G. Kennedy, E. A. Goodall and S. Kennedy. 1993b. Antioxidant enzyme activity in the muscle of calves depleted of vitamin E or selenium or both. Br. J. Nutr. 70:621-630.

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