Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Efficacy of rifampin and streptomycin in Sprague-Dawley ratsinfected with Brucella abortus

Brucella abortus 감염 흰쥐에서의 rifaampin과 streptomycin의 치료효과

  • Baek, Byeong-Kirl (College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, Chun-ki (College of Veterinary Medicine, Chonbuk National University) ;
  • Lim, Chae-woong (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, John-hwa (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Byeong-Soo (Department of Pet Science, Sohae College) ;
  • Lee, Sung-il (Toyama Medical and Pharmaceutical University) ;
  • Hur, Jin (College of Veterinary Medicine, University of Illinois) ;
  • Ibulaimu, Kakoma (College of Veterinary Medicine, University of Illinois)
  • Accepted : 2004.07.10
  • Published : 2004.09.30
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Abstract

This study was carried out to investigate the efficacy of rifampin with or without streptomycin in male Sprague-Dawley (SD) rats experimentally inoculated with Brucella abortus. Thirty rats were intraperitoneally inoculated with $1.0{\times}10^9$colony-forming units of B. abortus. They were divided into 3 groups by treatment with antibiotic. 10 rats in Group A were orally administrated with rifampin, 10 rats in Group B with rifampin orally and with streptomycin intramuscularly over 12 weeks starting at 1 week post infection (PI). A placebo recipient in Group C was inoculated with sterile saline without antibiotics. All animals were monitored by tube agglutination test (TAT) and AMOS-PCR to evaluate the efficiency of the antibiotics to B. abortus infection. The antibody titers in Groups A, B and C were 1:400, 1:400 and 1:800 as measured by TAT at the first week PI, respectively. The antibody titer in Group A decreased to 1:100 by the 13th week PI. That in the control group was observed as high antibody titer until 13th weeks PI, but the antibody response in Group B was low(1:50) from the 5th week to the 13th week PI. AMOS-PCR there was evidence of relapse of B. abortus in group A in liver and spleen specimens at the 13th week PI. B. abortus DNA was detected in Group C in liver and spleen specimens from the 1st week to 13th week PI by AMOS-PCR. However AMOS-PCR could not detect any organism in Group B from the 3rd week PI until the end of the study. This study demonstrated that administration of a combination of rifampin and streptomycin was more efficacious than administration of rifampin alone. A significant reduction in antibody titer was observed when a combination of 15 mg/kg/day of rifampin per os and 15 mg/kg/day streptomycin intramuscularly was used in comparison with the antibody of control group.

Keywords

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