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Roughage Energy and Degradability Estimation with Aspergillus oryzae Inclusion Using Daisy In vitro Fermentation

  • Chen, C.R. (Department of Animal Science, National Chung-Hsing University) ;
  • Yu, B. (Department of Animal Science, National Chung-Hsing University) ;
  • Chiou, P.W.S. (Department of Animal Science, National Chung-Hsing University)
  • 투고 : 2002.11.29
  • 심사 : 2003.09.16
  • 발행 : 2004.01.01

초록

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

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참고문헌

  1. AOAC. 1984. Official Methods of Analysis (14th Ed.). Association of Official Analytical Chemists, Washington, DC.
  2. Beharka, A. A. and T. G. Nagaraja. 1993. Effect of Aspergillus oryzae fermentation extract ($Amaferm^{\circledR}$) on in vitro fiber degradation. J. Dairy Sci. 76:812-818.
  3. Bertrand, J. A. and L. W. Grimes. 1997. Influence of tallow and Aspergillus oryzae fermentation extract in dairy rations. J. Dairy Sci. 80:1179-1184.
  4. Bruno-Soares, A. M., J. M. F. Abreu, C. V. M. Guedes and A. A. Diasda-Silva. 2000. Chemical composition, DM and NDF degradation kinetics in rumen of seven legume straw. Anim. Feed Sci. Technol. 83:75-80. https://doi.org/10.1016/S0377-8401(99)00113-3
  5. Chiou, P. W. S. 2002. Proceeding of feed manufacturing technology, a short course. 72-103, June 28, 2002, Taichung, Taiwan.
  6. Chiou, P. W. S., C. R. Chen, K. J. Chen and B. Yu. 1998. Wet brewers’ grains or bean curd pomace as partial replacement of soybean meal for lactating cows. Anim. Feed Sci. Technol. 74:123-134.
  7. Chiou, P. W. S., C. R. Chen and B. Yu. 2000. Effects of Aspergillus oryzae fermentation extract on in situ degradation of feedstuffs. Asian-Aust. J. Anim. Sci. 13:1076-1083.
  8. Chiou, P. W. S., C. R. Chen and B. Yu. 2002. Effects of Aspergillus oryzae fermentation extract on performance of lactating cows in the summer and winter in Taiwan. Asian-Aust. J. Anim. Sci. 15:382-389.
  9. Dewhurst, R. J., D. Hepper and A. J. F. Webster. 1995. Comparison of in sacco and in vitro techniques for estimating the rate and extent of rumen fermentation of a range of dietary ingredients. Anim. Feed Sci. Technol. 51:211-229.
  10. Fondevila, M., C. J. Newbold, P. M. Hotten and E. R. Orskov. 1990. A note on the effect of Aspergillus oryzae fermentation extract on the rumen fermentation of sheep given straw. Anim. Prod. 51:422-425.
  11. Frumholtz, P. P., C. J. Newbold and R. J. Wallace. 1989. Influence of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec). J. Agric. Sci. Camb. 113:169-172.
  12. Gomez-Alarcon, R. A., C. Dudas and J. T. Huber. 1990. Influence of cultures of Aspergillus oryzae on rumen and total tract digestibility of dietary components. J. Dairy Sci. 73:703-709.
  13. Holden, L. A. 1999. Comparison of methods of in vitro dry matter digestibility for ten feeds. J. Dairy Sci. 82:1791-1794. https://doi.org/10.3168/jds.S0022-0302(99)75409-3
  14. Huang, H. J., P. W. S. Chiou, C. R. Chen, J. K. Chiang and B. Yu. 1999. Effects of dried rice distillers’ and grain supplementation on the performance of lactating cows. Anim. Feed Sci. Technol. 77:303-315.
  15. Mabjeesh, S. J., M. Cohen and A. Arieli. 2000. In vitro methods for measuring the dry matter digestibility of ruminant feedstuffs: Comparison of methods and inoculum source. J. Dairy Sci. 83:2289-2294.
  16. Mandebvu, P., J. W. West, R. N. Gates and G. M. Hill. 1998. Effect of hay maturity, forage source, or neutral detergent fiber content on digestion of diets containing Tifton 85 bermudagrass and corn silage. Anim. Feed Sci. Technol. 73:281-290.
  17. Martin, S. A. and D. J. Nisbet. 1990. Effects of Aspergillus oryzae fermentation extract on fermentation of amino acids, bermudagrass and starch by mixed ruminal microorganisms in vitro. J. Anim. Sci. 68:2142-2149.
  18. McKenzie, R. A., B. J. Blaney, M. D. Connole and L. A. Fitzpatrick. 1981. Acute aflatoxicosis in calves fed peanut hay. Aust. Vet. J. 57:284-286.
  19. Nakamura, T., T. J. Klopfenstein and R. A. Britton. 1994. Evaluation of acid detergent insoluble nitrogen as an indicator of protein quality in nonforage proteins. J. Anim. Sci. 72:1043-1048.
  20. National Research Council. 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Washington, DC. Natl. Acad. Sci.
  21. Orskov, E. R. and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate passage. J. Agric. Sci. Camb. 92:499-503.
  22. Robinson, P. H. 2000. Predicting the energy value of feeds. Feedstuffs. 72:187.
  23. Rogers, J. A., H. R. Conrad, B. A. Dehority and J. A. Grubb. 1986. Microbial numbers, rumen fermentation, and nitrogen utilization of steers fed wet or dried brewer’s grains. J. Dairy Sci. 69:745-753.
  24. Saddler, J. N. 1986. Factors limiting the efficiency of cellulase enzymes. Microb. Sci. 3:84-87.
  25. SAS Institute. 1985. SAS User’s Guide, Version 5 ed. SAS Institute, Inc., Cary, NC.
  26. Tilly, J. M. and R. A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. J. Br. Grassl. Soc. 18:104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  27. Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant. 2nd ed. Cornell University Press, Ithaca, NY.
  28. Van Soest, P. J., J. B. Robbertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides on relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  29. Weidmeier, R. D., M. J. Arambel and J. L. Walters. 1987. Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility. J. Dairy Sci. 70:2063-2068.
  30. Weiss, W. P., D. O. Erickson, G. M. Erickson and G. R. Fisher. 1989. Barley distiller’s grains as a protein supplement for dairy cows. J. Dairy Sci. 72:980-987.
  31. Weiss, W. P. 1998. Estimating the available energy content of feeds for dairy cattle. J. Dairy Sci. 81:830-839. https://doi.org/10.3168/jds.S0022-0302(98)75641-3
  32. Weiss, W. P. 1993. Predicting energy value of feeds. J. Dairy Sci. 76:1802-1811. https://doi.org/10.3168/jds.S0022-0302(93)77512-8

피인용 문헌

  1. Effects of Aspergillus Oryzae Culture and 2-Hydroxy-4-(Methylthio)-Butanoic Acid on In vitro Rumen Fermentation and Microbial Populations between Different Roughage Sources vol.27, pp.9, 2014, https://doi.org/10.5713/ajas.2013.13742
  2. Adaptation and withdrawal of feeding dried Aspergillus oryzae fermentation product to dairy cattle and goats on in vitro NDF digestibility of selected forage sources vol.5, pp.2, 2004, https://doi.org/10.1093/tas/txab051