대한수의학회지 (Korean Journal of Veterinary Research)

  • 제44권1호
  • /
  • Pages.73-82
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  • 2004
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

가금티푸스 감염에 대한 키토산의 면역반응

Immunomodulatory effect on chitosan against Fowl typhoid infection

  • 조경오 (전남대학교 수의과대학) ;
  • 고흥범 (전남대학교 수의과대학) ;
  • 김계엽 (동신대학교 한의과대학)
  • Cho, Kyoung-Oh (College of Veterinary Medicine, Chonnam National University) ;
  • Koh, Hong-Bum (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Gye-Yeop (College of Oriental Medicine, Dongshin University)
  • 심사 : 2004.02.20
  • 발행 : 2004.03.31
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초록

Chitosan is similar in structure to cellulose and are the second most abundant polysaccharides in nature, comprising the horny substance in the exoskeletons of crabs, shrimp and insects as well as fungi. This study was conducted to access the effect of immunomodulation responses of chitosan(N-acetyl-${\beta}$-D-glucosamine) chicken infected with in Fowl typhoid(Salmonella gallinarum). One-day-old broiler chicks were divided into eight groups: The 1st group was inoculated intra-peritoneally with chitosan and challenged intra-peritoneally with S. gallinarum. The 2nd group was inoculated intra-peritoneally with chitosan. The 3rd group was feeding with chitosan and intra-peritoneally inoculated with cyclophosphamide and challenged intra-peritoneally S. gallinarum. The 4th group was feeding with chitosan and intra-peritoneally with cyclophosphamide. The 5th group was feeding with chitosan and challenged intra-peritoneally with S. gallinarum. The 6th group was feeding with chitosan. The 7th group was challenged intra-peritoneally with S. gallinarum. The 8th group was nontreated-uninfected control group. The results shows that $CD4^+$, $CD8^+$ and B lymphocyte in lymphoid organs of chickens treated with chitosan increased in especially $CD4^+$, $CD8^+$ lymphocytes (p<0.05). The group of feeding chitosan showed the significantly increased $CD4^+$, $CD8^+$ and B lymphocyte than inoculated intra-peritoneally with chitosan. As the result suggests that the feeding of chitosan induced immunostimulatant effect than the inoculation intra-peritoeally of chitosan.

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참고문헌

  1. 김영환, 김경희, 우용구, 경북지방 유래 츄백리 양성계에서의 균분리 및 혈청역가 추이. 대한수의학회지. 1997, 20, 19-26
  2. Aleksandersen, M., Hein, W. R., Landsvert, T. and McClure, S. Distribution of lymphocyte subsets in the large intestinal lymphoid follicles of lambs. Immunology. 1990, 70, 391-397
  3. Arai, K., Kinumaki, T. and Fugita, T. Toxicity of chitosan. Bull. Tokai Reg. Fish Res. Lab. 1968, 56, 86-94
  4. Blaxland, J. D., Sojka, W. J. and Smither, A. M. A study of Salmonella pullorum and Salmonella gallinarum strains isolated from field outbreaks of disease. J. Comp. Pahtol. Ther. 1956, 66, 270-277
  5. Bucy, R. P., Chen, C. H., Cihak, J., Losch, U. and Cooper, M. D. Avian T cells expressing gamma delta receptors localize in the splenic sinusoids and theintestinal epithelium. J. Immunol. 1988, 141, 2200-2205
  6. Chan, M. M., Chen, C. H., Ager, L. L. and Cooper, M. D. Differential expression of two T cell receptors, TcR1 and TcR2, on chichen lymphocyte. Eur. J. Immunol. 1988, 18, 539-543
  7. Gaudecker, B. V. Functional histolohy of the human hymus. Anat. Embryol. 1991, 183, 1-15
  8. Horsfall, D. J., Powley and Jenkins, C. R. The titer of bactericidal antibody against Salmonella gallinarum in chicks. Immunology. 1970, 18, 595-598
  9. Johnson, D. C., Keenum, K. G. and Peerry, H. P. A Fowl Typhoid Outbreak in a Chicken Breeder Flock. Avian Dis. 1977, 21, 716-719
  10. Kim, W. Y., Chang, Y. H., Park, K. Y., Kim, C. J., Shin, K. S. and Park, Y. H. Antimicrobial drug susceptibility and plasmid profiles of Salmonella species isolated from poultry. Korea J. Vet. Res. 1995, 35, 537-542
  11. Kina, K., Tamura, K. and Ishibashi, J. The fungicidal effect of chitosan of fungi of varying cell wall composition. Japan Analist. 1974, 23, 1902-1100
  12. Klasing, K. C. Nutritional modulation of resistance to infectious diseases. Poultry Sci. 1998, 77, 1119-1125
  13. Klesius, P., Johnson, W. and Kramer, T. Delayed wattle reaction as a measure of cell-mediated immunity in the chicken. Poultry Sci. 1977, 56, 249-256
  14. Kosaka, Kaneko Y. and Nakacla, Y. Effect of chitosan Implatation on activation of canine macrophages and polymorphonuclear cells after surgical stress. J. Vet. Med. Sci. 1996. 58, 957-963
  15. Minami, S., Egawa, T. and Ohika, J. Effects of chitosan with Intramammary administration of phagocytes in under secretion. J. Jpn. Vet. Med. Assoc. 1997, 50, 143
  16. Muzzarelli, R., Tarsi, R. and Filippini, O. Antimicrobial properties of N carboxybutyl chitosan. Antimicrob. Agnets Chemother. 1990, 34, 2019-2023
  17. Nishimura, K., Nishimura, S. and Nishi, N. Immunological activity of chitin and its derivatives. Vaccine. 1984, 2, 93-99
  18. Nishi, N. Immunological activity of chitin and its derivatives. Vaccine. 1984, 2, 93-99
  19. Okamoto, Y., Minami, S. and Matsuhashi, A. Effects of chitosan on bovine wound healing. J. Jpn. Vet. Med. Assoc. 1996, 49, 22
  20. Peluso, G., Petillo, O. and Ranieri, M. Chitosanmediated stimulation of marophage function. Biomaterials. 1994, 15, 1215-1220
  21. Poppema, S., Bhan, A. K., Reinherz, E. L., MaCluskey, R. T. and Schlossman, S. F. Distribution of T cell subsets in human lymph nodes. J. Exp. Med. 1981, 153, 30-41
  22. Razdan, A., Pettersson, D. and Pettersson J. Bwiler, chicken body weights, feed intakes, plasma lipid andsmall-intestinal bile acid concentrations in response tofeeding of chitosan and pectin. Br. J. Nutr. 1997, 78, 283-291
  23. Shibata, Y., Foster, L. A., Metzger, W. J. and Myrvik, Q. N. Alveola Macrophage Priming by Intravenous Administration of Chitin Particles, polymers of NAcetyl-D-Glucosamine, in mice. Am. Soc. Microbiol. 1997, 65, 1734-1741
  24. Smith, W. H. and Tucker, J. F. The virulence of Salmonella strains for chickens. J. Hyg. 1980, 84, 479-488
  25. Sugimura, M. and Hashimoto, Y. T-B dependent areas and movement of macrophages in avian lymphatic tissue. Igakunoayumi. 1981. 119, 867-879
  26. Suzuki, K., Tokoro, A. and Okawa, Y. Effect of NAcetyl Chito-Oligosaccharides on Activation of Phagocytes. Microbiol Immunol. 1986, 30, 777-787
  27. Sudurshan, N. R., Hoove, D. G. and Knorr, D. Antibacterial Action of chitosan. Food Biotechnol. 1992, 6, 257-272
  28. Swenson, M. J. and Dukes, H. H. Physiology of domestic animals. pp. 35-40, 10th ed. Cornell Univ. Press. Ithaca. 1984
  29. Turnbull, P. C. B. and Snoeyenbos, G. H. Experimental Salmonellosis in the Chicken. 2. Fate of a Temperature-Sensitive filamentous Mutant. AvianDis. 1973, 18, 178-185
  30. Usami, Y. and Okamoto, Y. Minamis chitosan induce migration of bovine polymorphonuclear cells. J. Vet. Med. Sci. 1994, 56, 761