Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Analysis of the Anaerobic Bacterial Community in the Earthworm (Eisenia fetida) Intestine

Shin, Kwang-Hee;Yi, H.;Chun, Jong-Sik;Cha, Chang-Jun;Kim, In-Seon;Hur, Hor-Gil

  • Published : 2004.09.30
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Abstract

Intestinal microbial community structure of earthworm Eisenia fetida was investigated based on 16S rDNA analysis. One hundred different colonies grown on Brain Heart Infusion medium were randomly isolated. Through partial sequence analysis of PCR-amplified 16S rDNA, earthworm intestinal bacteria (EIB) were divided into eight groups, which were further divided into subgroups. Groups EIB 2, EIB 3, EIB 4, EIB 5, EIB 6, EIB 7-1, and EIB 8 showed over 97% similarities to Clostridium bifermentans, C. butyricum, C. glycolicum, C. celerecrescens, C. lituseburense, Staphylococcus epidermidis, and Propionibacterium acnes, respectively. Group EIB 1 consisting of six subgroups, showed unique pyretic line, found to be most closely related to C. subterminale with 90-95% similarity. Subgroup EIB 7-2 showed 93% similarity to S. epidermidis. Among 100 strains, intestinal microbial community consisted of 49, 13, 13, 5, 4, 2, 11, and 3% EIB 1, EIB 2, EIB 3, EIB 4, EIB 5, EIB 6, EIB 7 and EIB 8, respectively, indications that group EIB 1 was dominant bacterial group in earthworm intestinal bacterial community. Considering earthworm plays key role in improving physical and chemical properties of soil, this study provides valuable information on bacterial community structure of intestine of these ecologically important organisms.

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