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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Efficient Transduction with Recombinant Adenovirus in EBV-transformed B Lymphoblastoid Cell Lines

  • Kim, Hye-Jin (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Cho, Hyun-Il (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Han, Yoon-Hee (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Park, Soo-Young (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Kim, Dong-Wook (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Lee, Dong-Gun (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Kim, Jee-Hoon (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Shin, Wan-Shik (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Paik, Soon-Young (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Kim, Chun-Choo (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Hong, Young-Seon (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Kim, Tai-Gyu (Department of Microbiology and Immunology, Clinical Institute of St. Mary's Hospital, College of Medicine, The Catholic University of Korea)
  • Published : 2004.05.31
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Abstract

The Epstein-Barr-transformed B lymphoblastoid cell lines, LCL, which express antigens, are potential antigen-presenting cells (APCs) for the induction of cytotoxic T lymphocytes in vitro. However, transfecting LCL with subsequent selection by antibiotics is notoriously difficult because the plating efficiencies of LCL are reported to be 1% or less. Therefore, this study investigated the optimal conditions for increasing the transduction efficiency of a recombinant adenovirus to LCL for use as a source of APCs. The transduction efficiencies were < 13% (SD $\pm$ 2.13) at a multiplicity of infection (MOI) of 100, while it was increased to 28% (SD $\pm$ 9.43) at an MOI of 1000. Moreover, its efficiencies to LCL that expressed the coxsackie adenovirus receptor were increased to 60% (SD $\pm$ 6.35) at an MOI of 1000, and were further increased to 70% (SD $\pm$ 4.56) when combined with the centrifugal method. The cationic liposome or anionic polymer had no effect on the transduction efficiency when compared to that of the centrifugal method. These results may be used as a convenient source of target cells for a CTL assay and/or autologous APCs for the induction of the in vitro CTL responses that are specific to viral and tumor antigens.

Keywords

References

  1. Bahnson, A. B., Dunigan, J. T., Baysal, B. E., Mohney, T., Atchison, R. W., Nimgaonkar, M. T., Ball, E. D. and Barranger, J. A. (1995) Centrifugal enhancement of retroviral mediated gene transfer. J. Virol. Methods 54, 131-143. https://doi.org/10.1016/0166-0934(95)00035-S
  2. Bergelson, J. M., Cunningham, J. A., Droguett, G., Kurt-Jones, E. A., Krithivas, A., Hong, J. S., Horwitz, M. S., Crowell, R. L. and Finberg, R. W. (1997) Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5. Science 275, 1320-1323. https://doi.org/10.1126/science.275.5304.1320
  3. Bill, S. and Willie, M. (1977) Clonal transformation of adult human leukocytes by Epstein-Barr virus. J. Virol. 23, 503-508.
  4. Buschle, M., Brenner, M. K., Chen, I. S., Drexler, H. G., Gignac, S. M. and Rooney C. M. (1990) Transfection and gene expression in normal and malignant primary B lymphocytes. J. Immunol. Methods 133, 77-85. https://doi.org/10.1016/0022-1759(90)90321-L
  5. Byk, T., Haddada, H., Vainchenker, W. and Louache, F. (1998) Lipofectamine and related cationic lipids strongly improve adenoviral infection efficiency of primitive human hematopoietic cells. Hum. Gene Ther. 9, 2493-2502. https://doi.org/10.1089/hum.1998.9.17-2493
  6. Clark, P. R., Stopeck, A. T., Brailey, J. L., Wang, Q., McArthur, J., Finer, M. H. and Hersh, E. M. (1999) Polycations and cationic lipids enhance adenovirus transduction and transgene expression in tumor cells. Cancer Gene Ther. 6, 437-446. https://doi.org/10.1038/sj.cgt.7700074
  7. Curiel, D. T., Wagner, E., Cotten, M., Birnstiel, M. L., Agarwal, S., Li, C. M., Loechel, S. and Hu, P. C. (1992) High-efficiency gene transfer mediated by adenovirus coupled to DNApolylysine complexes. Hum. Gene Ther. 3, 147-154. https://doi.org/10.1089/hum.1992.3.2-147
  8. Curiel, T. J., Wong, J. T., Gorczyca, P. F., Schooley, R. T. and Walker, B. D. (1993) CD4+ human immunodeficiency virus type 1 (HIV-1) envelope-specific cytotoxic T lymphocytes derived from the peripheral blood cells of an HIV-1-infected individual. AIDS Res. Hum. Retroviruses 9, 61-68. https://doi.org/10.1089/aid.1993.9.61
  9. Curiel, T. J., Cook, D. R., Bogedain, C., Jilg, W., Harrison, G. S., Cotton, M., Curiel, D. T. and Wagner, E. (1994) Efficient foreign gene expression in Epstein-Barr virus-transformed human B-cells. Virology 198, 577-585. https://doi.org/10.1006/viro.1994.1069
  10. DeMatteo, R. P., Chu, G., Ahn, M., Chang, E., Burke, C., Raper, S. E., Barker, C. F. and Markmann, J. F. (1997) Immunologic barriers to hepatic adenoviral gene therapy for transplantation. Transplantation 63, 315-319 https://doi.org/10.1097/00007890-199701270-00024
  11. Dietz, A. B. and Vuk-Pavlovic, S. (1998) High efficiency adenovirus-mediated gene transfer to human dendritic cells. Blood 91, 392-398.
  12. Dustin, M. L., Staunton, D. E. and Springer, T. A. (1988) Supergene families meet in the immune system. Immunol. Today 9, 213-215. https://doi.org/10.1016/0167-5699(88)91216-9
  13. Eloit, M., Gilardi-Hebenstreit, P., Toma, B. and Perricaudet, M. (1990) Construction of a defective adenovirus vector expressing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine. J. Gen. Virol. 71, 2425-2431. https://doi.org/10.1099/0022-1317-71-10-2425
  14. Fasbender, A., Zabner, J., Zeiher, B. G. and Welsh, M. J. (1997) A low rate of cell proliferation and reduced DNA uptake limit cationic lipid-mediated gene transfer to primary cultures of ciliated human airway epithelia. Gene Ther. 4, 1173-1180. https://doi.org/10.1038/sj.gt.3300524
  15. George, M., Thomas, S., Hermann, L., Daphne, S. and Muriel, L. (1972) Epstein-Barr virus: Transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69, 383-387. https://doi.org/10.1073/pnas.69.2.383
  16. Ho, W. Z., Cherukuri, R., Ge, S. D., Cutilli, J. R., Song, L., Whitko, S. and Douglas, S. D. (1993) Centrifugal enhancement of human immunodeficiency virus type 1 infection and human cytomegalovirus gene expression in human primary monocyte/macrophages in vitro. J. Leukoc. Biol. 53, 208-212.
  17. Huang, S., Endo, R. I. and Nemerow, G. R. (1995) Upregulation of integrins alpha v beta 3 and alpha v beta 5 on human monocytes and T lymphocytes facilitates adenovirus-mediated gene delivery. J. Virol. 69, 2257-2263.
  18. Hudson, J. B. (1988) Further studies on the mechanism of centrifugal enhancement of cytomegalovirus infectivity. J. Virol. Methods 19, 97-108. https://doi.org/10.1016/0166-0934(88)90153-X
  19. Hureley, E. and Thorley-Lawson, D. (1988) B cell activation and the establishment of Epstein-Barr virus latency. J. Exp. Med. 168, 2059-2075. https://doi.org/10.1084/jem.168.6.2059
  20. Jerome, K. R., Bu, D. and Finn, O. J. (1992) Expression of tumor-associated epitopes on Epstein-Barr virus-immortalized B-cells and Burkitt's lymphomas transfected with epithelial mucin complementary DNA. Cancer Res. 52, 5985-5990.
  21. Kim, D. J., Jang, H. J. Pyun, Y. R. and Kim, Y. S. (2002) Cloning, expression, and characterization of thermostable DNA polymerase from Thermoanaerobacter youseiensis. J. Biochem. Mol. Biol. 35, 320-329. https://doi.org/10.5483/BMBRep.2002.35.3.320
  22. Lanuti, M., Kouri, C. E., Force, S., Chang, M., Amin, K., Xu, K., Blair, I., Kaiser, L. and Albelda, S. (1999) Use of protamine to augment adenovirus-mediated cancer gene therapy. Gene Ther. 6, 1600-1610. https://doi.org/10.1038/sj.gt.3300987
  23. Lee, S. M., Lee, K. H., Kim, H. D., Lee, J. H., Lee, J. S. and Kim, J. (2001) Co-expression of MDR1 and HLA-B7 Genes in a mammalian cell using a retrovirus. J. Biochem. Mol. Biol. 34, 176-181.
  24. Leon, R. P., Hedlund, T., Meech, S. J., Li, S., Schaack, J., Hunger, S. P., Duke, R. C. and DeGregori, J. (1998) Adenoviralmediated gene transfer in lymphocytes. Proc. Natl. Acad. Sci. USA 95, 13159-13164. https://doi.org/10.1073/pnas.95.22.13159
  25. Livingston, P. G., Kurane, I. and Ennis, F. A. (1997) Use of Epstein-Barr virus-transformed, autologous B-lymphoblastoid cells as antigen-presenting cells for establishment and maintenance of dengue virus-specific, human cytotoxic T lymphocyte clones. J. Virol. Methods 67, 77-84. https://doi.org/10.1016/S0166-0934(97)00082-7
  26. McFarland, E. J., Curiel, T. J., Schoen, D. J., Rosandich, M. E., Schooley, R. T. and Kuritzkes, D. R. (1993) Cytotoxic T lymphocyte lines specific for human immunodeficiency virus type 1 Gag and reverse transcriptase derived from a vertically infected child. J. Infect. Dis. 167, 719-723. https://doi.org/10.1093/infdis/167.3.719
  27. Miller, A.D. (1992) Human gene therapy comes of age. Nature 357, 455-460. https://doi.org/10.1038/357455a0
  28. Mueller, D. L., Jenkins, M. K. and Schwartz, R. H. (1989) Clonal expansion versus functional clonal inactivation: a costimulatory signaling pathway determines the outcome of T cell antigen receptor occupancy. Annu. Rev. Immunol. 7, 445-480. https://doi.org/10.1146/annurev.iy.07.040189.002305
  29. Nishimura, N., Nishioka, Y., Shinohara, T., Ogawa, H., Yamamoto, S., Tani, K. and Sone, S. (2001) Novel centrifugal method for simple and highly efficient adenovirus-mediated green fluorescence protein gene transduction into human monocytederived dendritic cells. J. Immunol. Methods 253, 113-124. https://doi.org/10.1016/S0022-1759(01)00360-X
  30. Nissol, K. and Ponten, J. (1975) Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer 15, 321-341. https://doi.org/10.1002/ijc.2910150217
  31. Ohlen, C., Bejarano, M. T., Gronberg, A., Torsteinsdottir, S., Franksson, L., Ljunggren, H. G., Klein, E., Klein, G. and Karre, K. (1989) Studies of sublines selected for loss of HLA expression from an EBV-transformed lymphoblastoid cell line. Changes in sensitivity to cytotoxic T cells activated by allostimulation and natural killer cells activated by IFN or IL-2. J. Immunol. 142, 3336-3341.
  32. Reimann, J., Heeg, K., Wagner, H., Keller, G. and Wagner, E. F. (1986) Introduction of a selectable gene into murine Tlymphoblasts by a retroviral vector. J. Immunol. Methods 89, 93-101. https://doi.org/10.1016/0022-1759(86)90036-0
  33. Richter, W., Eiermann, T. H. and Scherbaum, W. A. (1990) Effect of cytokines on proliferation of Epstein-Barr virus-transformed B lymphocytes. Hybridoma 9, 1-8. https://doi.org/10.1089/hyb.1990.9.1
  34. Shankar, P., Fabry, J. and Lieberman, J. (1995) A simple method to selectively expand HIV-1 specific cytotoxic T lymphocytes in vitro. Immunol. Invest. 24, 489-497. https://doi.org/10.3109/08820139509066845
  35. Stockwin, L. H., Matzow, T., Georgopoulos, N. T., Stanbridge, L. J., Jones, S. V., Martin I. G., Blair-Zajdel, M. E. and Blair, G. E. (2002) Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer. J. Immunol. Methods 259, 205-215. https://doi.org/10.1016/S0022-1759(01)00510-5
  36. Sun, Q., Pollok, K. E., Burton, R. L., Dai, L. J., Britt, W., Emanuel, D. J. and Lucas, K. G. (2001) Simultaneous ex vivo expansion of cytomegalovirus and Epstein-Barr virus-specific cytotoxic T lymphocytes using B-lymphoblastoid cell lines expressing cytomegalovirus pp65. Blood 94, 3242-3450.
  37. van Baalen, C. A., Klein, M. R., Geretti, A. M., Keet, R. I., Miedema, F., van Els, C. A. and Osterhaus, A. D. (1993) Selective in vitro expansion of HLA class I-restricted HIV-1 Gag-specific CD8+ T cells: cytotoxic T-lymphocyte epitopes and precursor frequencies. AIDS 7, 781-786. https://doi.org/10.1097/00002030-199306000-00004
  38. Wagner, E., Zatloukal, K., Cotten, M., Kirlappos, H., Mechtler, K., Curiel, D. T. and Birnstiel, M. L. (1992) Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc. Natl. Acad. Sci. USA 89, 6099-6103. https://doi.org/10.1073/pnas.89.13.6099
  39. Wang, K., Huang, S., Kapoor-Munshi, A. and Nemerow, G. (1998) Adenovirus internalization and infection require dynamin. J. Virol. 72, 3455-3458.

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