Evaluation of clinical usefulness of reference interval of some selected hematological parameters in canine blood

  • Pak, Son-Il (Department of Veterinary Medicine, Kangwon National University) ;
  • Han, Ho-Jae (College of Veterinary Medicine, Chonnam National University)
  • 심사 : 2004.05.10
  • 발행 : 2004.12.30

초록

To estimate the source of variance components for some hematological parameters and assess the utility of the conventional population-based reference interval, this study computed index of individuality for blood samples, which were from 13 dogs drawn once weekly for 4 consecutive weeks. Results were subjected to nested analysis of variance. For all parameters measured between-dog variations were greater than within-dog variation. Except for the parameters RBC and MCHC the index of individuality was <1.4. The low reliability coefficient and high index of individuality of ${\leq}0.8$ were found for the majority of hematological parameters. In practical term, the present study indicated that use of hemogram profiles alone in the evaluating clinical state of a single patient should be avoided because of their physiological or natural random variations, and that comparing a single measurement on the blood analytes from an individual dog to the conventional population-based reference range may be too insensitive to detect any significant changes in the blood components of that particular dog. A single measurement may not characterize an individual's average concentration of the parameters even shortterm period.

키워드

참고문헌

  1. Chambless, L. E., McMahon, R., Wu, K., Folsom, A., Finch, A. and Shen, Y. L. Short-term intraindividual variability in hemostasis factors: the ARIC study. Ann.Epidemiol. 1992, 2, 723-733
  2. Eckfeldt, J. H., Chambless, L. E. and Shen, Y. Shortterm, within-person variability in clinical chemistry results: experience from the atherosclerosis risk in communities study. Arch. Pathol. Lab. Med. 1994, 118, 496-500
  3. Fraser, C. G. and Harris, E. K. Generation and application of data on biological variation in clinical chemistry. Crit. Rev. Clin. Lab. Sci. 1989, 27, 409-437
  4. Fraser, C. G. and Hearne, C. R. Components of variance of some plasma constituents in patients with myocardial infarction. Ann. Clin. Biochem. 1982, 19,431-434
  5. Garg, U. C., Zheng, Z. J., Folsom, A. R., Moyer, Y. S., Tsai, M. Y., McGovern, P. and Eckfeldt, J. H. Short-term and long-term variability of plasma homocysteine measurement. Clin. Chem. 1997, 43, 141-145
  6. Graybill, F. A. and Wang, C. M. Confidence intervals for proportions of variability in two-factor nested variance component models. J.Am. Stat. Assoc. 1979, 74, 368-374
  7. Harris, E. K. Effects of intra- and interindividual variation on the appropriate use of normal ranges. Clin. Chem. 1974, 20, 1535-1542
  8. H$\"{o}$lzel, W. G. E. Intra-individual variation of some analytes in serum from patients with insulin-dependent diabetes mellitus. Clin. Chem. 1987, 33, 57-61
  9. Jensen, A. L., Houe, H. and Nielsen, C. G. Critical differences of clinical-chemical parameters in blood from Red Danish dairy cows. Res. Vet. Sci. 1992, 52, 86-89
  10. Jensen, A. L. Critical differences of clinical chemical parameters in blood from dogs. Res. Vet. Sci. 1993, 54, 10-14
  11. Jensen, A. L., Koch, J. and Wenck, A. Estimates of components of variance of the rectal temperature in clinically healthy dogs in a hospital environment. J. Vet.Med. A. 1994, 41, 717-721
  12. Marca, M. C. and Loste, A. Glycosylated haemoglobin in dogs: study of critical difference value. Res. Vet. Sci. 2001, 71, 115-117
  13. Queralto, J. M., Boyd, J. C. and Harris, E. K. On the calculation of reference change values, with examples from a long-term study. Clin. Chem. 1993, 39, 1398-1403
  14. Pascoe, P. J., Gallagher, C. S. and Fraser, C. G. Components of biological variation of some serum analytes in hospitalized pregnant women. Clin. Chem.1984, 30, 583-584
  15. Ross, J. W. Evaluation of precision. In: Werner M, ed. Handbook of clinical chemistry. pp. 391-422. CRC Press, Boca Raton, 1982
  16. Snedecor, G. W. and Cochrane, W. G. Statistical methods. pp. 379-384. 7th ed. Iowa State University Press, Ames, 1980