Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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The Algicidal Effect of Antimicrobial Peptide, Apidaecin Ib

항균성 펩타이드인 Apidaecin Ib의 살조효과

  • Published : 2004.08.30
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Abstract

Apidaecin Ⅰb, an antimicrobial peptide isolated from lymph fluid of the honeybee (Apis mellifera), is a basic non-helical peptide composed of eighteen amino acid residues. In this study, we have investigated the algicidal effect of Apidaecin Ⅰb against harmful algae blooms (HABs) causative Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides and Gymnodinium catenatum. The algicidal effect of Apidaecin Ⅰb showed in the concentration of 12.5 $\mu{g}/mL$ to 50$\mu{g}/mL$ against 4 HAB species and observed cell lysis or cell ecdysis by microscopy. Apidaecin Ⅰb reacted more sensitive to C. marina than A. tamarense, C. polykrikoides and G. catenatum. The algicidal study of Apidaecin Ⅰb against HABs will provides much insight into development of new algicidal substances.

꿀벌 (Apis mellifera)의 lymph 액으로부터 정제된 항균성 펩타이드인, Apidaecin Ⅰb는 18개의 아미노산 잔기로 구성된 염기성 non-helical 펩타이드이다. 본 연구에서는 우리나라 연안의 적조 (HABs, harmful algal blooms)를 일으키는 4종의 적조생물 (Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides 및 Gymnodinium catenatum)에 대한 Apidaecin Ⅰb의 살조효과를 조사하였다. Apidaecin Ⅰb의 4종의 적조생물에 대한 살조효과는 12.5$\mu{g}/mL$부터 50$\mu{g}/mL$에서 세포의 lysis 또는 ecdysis와 같은 형태로 현미경으로 관찰할 수 있었다. 또한 Apidaecin Ⅰb는 C. marina에 대해서 A. tamarense, C. polykrikorides 및 G. catenatum보다 더욱 강한 살조효과를 나타내었다. 이러한 HABs에 대한 Apidaecin Ⅰb의 살조효과 연구는 새로운 살조물질을 개발하기 위한 자료가 될 것으로 생각된다.

Keywords

References

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