Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Extenders and Temperatures on Sperm Viability and Fertilizing Capacity of Harbin White Boar Semen during Long-term Liquid Storage

  • Zhou, J.B. (College of Life Science, North-east Agricultural University) ;
  • Yue, K.Z. (College of Life Science, North-east Agricultural University) ;
  • Luo, M.J. (Laboratory for Animal Reproduction and Embryology College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Chang, Z.L. (Laboratory for Animal Reproduction and Embryology College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Liang, H. (College of Life Science, North-east Agricultural University) ;
  • Wang, Z.Y. (Laboratory for Animal Reproduction and Embryology College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Tan, J.H. (Laboratory for Animal Reproduction and Embryology College of Animal Science and Veterinary Medicine, Shandong Agricultural University)
  • Received : 2004.02.16
  • Accepted : 2004.06.12
  • Published : 2004.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study the effect of extenders and temperatures on sperm viability and fertilizing capacity of boar sperm during long-term storage was investigated. Acrosomal integrity, membrane integrity, motility and hypo-osmotic resistance were evaluated by fluorescence and light microscopy. An in vitro fertilization test was performed to assess the fertilizing capacity of stored spermatozoa. The five diluents tested were ranked according to their ability to maintain sperm functional parameters and Zorlesco (ZO) extender with BSA or with PVA instead of BSA produced the best results. Zorlesco extender substituted with PVA (ZO+PVA) was found to maintain motility both at 15 and 20$^{\circ}C$. within 5 days of storage, but the quality of semen stored at 15$^{\circ}C$ decreased thereafter as compared to semen stored at 20$^{\circ}C$ Semen stored at 5$^{\circ}C$ demonstrated rapid loss of motility already within 24 h. Both fertilization and cleavage of semen stored at 20$^{\circ}C$ in ZO substituted with PVA instead of BSA did not change significantly until day 8 of storage. It is therefore concluded that PVA can be used to substitute for BSA and 20$^{\circ}C$ was more suitable than 15$^{\circ}C$ for boar semen storage, and in vitro fertilizing capacity of spermatozoa was maintained for at least 8 days in ZO+PVA at 20$^{\circ}C$.

Keywords

References

  1. Amann, R. P. 1989. Can the fertility potential of a seminal sample be predicted accurately? J. Androl. 10:89-98.
  2. Brinster, R. L. 1963. A method for in vitro cultivation of mouse ova from two-cell to blastocyst. Exp. Cell. Res. 32:205-208. https://doi.org/10.1016/0014-4827(63)90093-4
  3. Cheng, W. T. K. 1988. Preservation of boar semen at $15^{\circ}C$. J. Chinese Soc. Anim. Sci. 14:339-350.
  4. Cheon, Y. M., H. K. Kim, C. B. Yang, Y. J. Yi and C. S. Park. 2002. Effect of season influencing semen characteristics, frozenthawed sperm viability and testosterone concentration in Duroc boars, Asian-Aust. J. Anim. Sci. 17:612-616.
  5. Chyr, S. C., M. C. Wu, Y. M. Su and J. Y. Tsai. 1980. The study of semen extenders and some reproductive performance of boar. J. Chinese Soc. Anim. Sci. 9:133-143.
  6. DasGupta, S., C. L. Mills and L. R. Fraser. 1993. Ca (2+)-related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay. J. Reprod. Fertil. 99:135-143.
  7. De Leeuw, A. M., D. H. G. den Daas and H. Woelders. 1991. The fix vital stain method: simultaneous determination of viability and acrosomal status of bovine spermatozoa. J. Androl. 12:112-118.
  8. Fraser, L. R., L. R. Abeydeera and K. Niwa. 1995. Ca (2+)-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol. Reprod. Dev. 40:233-241.
  9. Gottardi, L., L. Brunel and L. Zanelli. 1980. New dilution media for artificial insemination in the pig. The 9th International Congress of Animal Reproduction, Madrid. Vol. 5:49-53.
  10. Hafez, E. S. E. 1987. Semen evaluation. In: Reproduction in Farm Animals (5th Ed.). (Ed. E. S. E. Hafez). Lea and Febiger. pp. 455-480.
  11. Harrison, R. A. P., H. M. Dott and G. C. Foster. 1982. Bovine serum albumin, sperm motility, and the dilution effect. J. Exp. Zool. 222:81-88.
  12. Jeyendran, R. S., H. H. Van der Ven, M. Perez-Pelaez, B. G. Crabo and L. J. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70:219-228.
  13. Johnson, L. A. 1998. Current developments in swine semen: preservations, artificial insemination and sperm sexing. In: Proceedings of the 15th International Pig Vet Science Congress. Vol. 1:225-229.
  14. Johnson, L. A., J. G. Aalbers and H. J. G. Grooten. 1988. Artificial insemination of swine: Fecundity of boar semen stored in Beltsville TS BTS, Modified Modena MM, or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49-55.
  15. Johnson, L. A., K. F. Weitze, P. Fiser and W. M. Maxwell. 2000. Storage of boar semen. Anim Reprod Sci. 62:143-172.
  16. Mattioli, M., M. L. Bacci, G. Galeati and E. Seren. 1989. Development competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:1201-1207.
  17. Nagai, T., K. Niwa and A. Iritani. 1984. Effect of sperm concentration during preincubation in a defined medium on fertilization in vitro of pig follicular oocytes. J. Reprod. Fertil. 70: 271-275.
  18. Naito, K., Y. Fukada and I. Ishibashi. 1989. Developmental ability of porcine ova matured in porcine follicular fluid in vitro and fertilized in vitro. Theriogenology 31:1049-1057.
  19. Paulenz, H., L. Soderquist, R. Perez-Pe and K. A. Berg. 2002. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 57:823-836.
  20. Plisko, N. T. 1965. Method of prolonging the viability and fertilizing capacity of boar spermatozoa. Svinovodstvo 9:37-41 (in Russian).
  21. Polge, C. 1956. Artificial insemination in pigs. Vet. Rec. 68:62-76.
  22. Pursel, V. G. and L. A. Johnson. 1975. Freezing of boar spermatozoa: Fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40:99-102.
  23. Pursel, V. G., L. A. Johnson and L. L. Schulman. 1973. Fertilizing capacity of boar semen stored at 15 C. J. Anim. Sci. 37:532-535.
  24. Saling, P. M. and B. T. Storey. 1979. Mouse gamete interactions during fertilization in vitro: Chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction. J. Cell Biol. 83:544-555.
  25. Schill, W. B., E. Topfer-Petersen and E. Heissler. 1988. The sperm acrosome: functional and clinical aspects. Hum. Reprod. 3:139-145.
  26. Soede, N. M., C. C. H. Wetzels, W. Zondag, M. A. I. de Koning and B. Kemp. 1995. Effect of time of insemination relative to ovulation, as determined by ultrasonography, on fertilization rate and accessory sperm count in sows. J. Reprod. Fert. 104:99-106.
  27. Vazquez, J. M., E. A. Martinez, P. Martinez, C. Garcia-Artiga and J. Roca. 1997. Hypo-osmotic swelling of boar spermatozoa compared to other methods for analyzing the sperm membrane. Theriogenology 47:913-922.
  28. Waberski, D., K. F. Weitze, C. Lietmann, W. Lubbert zur Lage, F. Bortolozzo, T. Willmen and R. Petzoldt. 1994b. The initial fertilizing capacity of long-term-stored liquid boar semen following pre-and postovulatory insemination. Theriogenology 41:1367-1377.
  29. Waberski, D., K. F. Weitze, D. Rath and H. P. Sallmann. 1989. Effect of bovine serum albumin and zwitterionic buffers on stored liquid boar semen. Zuchthygiene 24:128-133.
  30. Waberski, D., S. Meding, G. Dirksaen, K. F. Weitze, C. Lewiding and R. Hahn. 1994a. Fertility of long term-stored boar semen: influence of extender Androhep and Kiev, storage time and plasma droplets in the semen. Anim. Reprod. Sci. 36:145-151.
  31. Wang, W. H., L. R. Abeydeera, L. R. Fraser and K. Niwa. 1995. Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen-thawed ejaculated boar spermatozoa incubated in a protein-free chemically defined medium. J. Reprod. Fertil. 104:305-313.
  32. Ward, C. R. and B. T. Storey. 1984. Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Dev. Biol. 104:287-296.
  33. Watson, P. F. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7:871-891. https://doi.org/10.1071/RD9950871
  34. Weitze, K. F. 1990. The use of long-term extender in pig AI-a view of the international situation. Pig News Information. 11:23-26.
  35. Yi, Y. J., H. J. Ko, S. H. Lee, C. B. Yang, D. S. Son, H. K. Kim and C. S. Park. 2004a. In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws. Asian-Aust. J. Anim. Sci. 17:164-167.
  36. Yi, Y. J., S. H. Lee and C. S. Park. 2004b. Comparison of semen characteristics, frozen-thawed sperm viability, testosterone concentration and embryo development between Yorkshire Boar A and B. Asian-Aust. J. Anim. Sci. 17:612-616.

Cited by

  1. Liquid Storage of Goat Semen in Chemically Defined Extenders vol.44, pp.5, 2009, https://doi.org/10.1111/j.1439-0531.2008.01071.x
  2. Protocol Optimization for Long-Term Liquid Storage of Goat Semen in a Chemically Defined Extender vol.44, pp.6, 2009, https://doi.org/10.1111/j.1439-0531.2008.01101.x
  3. Influence of Osmolality of Complete Semen Extender on Motion Characteristics of Frozen-thawed Ram Spermatozoa vol.19, pp.12, 2004, https://doi.org/10.5713/ajas.2006.1716
  4. The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins vol.19, pp.4, 2004, https://doi.org/10.5713/ajas.2006.486
  5. Evaluation of Boar Sperm Viability by MTT Reduction Assay in Beltsville Thawing Solution Extender vol.21, pp.4, 2004, https://doi.org/10.5713/ajas.2008.70480