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Activity of Some Hepatic Enzymes in Schistosomiasis and Concomitant Alteration of Arylsulfatase B

  • Balbaa, Mahmoud (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • El-Kersh, Mohamed (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • Mansour, Hamdy (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • Yacout, Galila (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • Ismail, Mohamed (Department of Bioscience and Technology, Institute of Graduate Studies and Research, Alexandria University) ;
  • Malky, Ahmed (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • Bassiouny, Khaled (Genetic Engineering, Biotechnology Research Institute, Monifia University) ;
  • Abdel-Monem, Nihad (Department of Biochemistry, Faculty of Science, Alexandria University) ;
  • Kandeel, Kamal (Department of Biochemistry, Faculty of Science, Alexandria University)
  • Published : 2004.03.31
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Abstract

The levels of arylsulfatases A and B, $\alpha$-amylase, aspartate transcarbamylase, and $\gamma$-glutamyl transpeptidase were investigated during the infection of mice with schistosoma mansoni. This infection caused a significant (p<0.001) increase in the activity of hepatic arylsulfatase B (ASB), aspartate transcarbamylases and $\gamma$-glutamyl transpeptidase. A non-significant difference occurred for $\alpha$-amylase (p<0.3) and arylsulfatase A (p>0.5) when compared to the control. The specific activity of hepatic ASB was progressively increased with the progression of the Schistosoma-infection. Moreover, the kinetic studies of hepatic ASB in Schistosoma-infection showed that a slight decrease in the value of $K_m$ and about a 40% increase in $V_{max}$ when compared to the control. In addition, the pH optimum of hepatic ASB was altered from 6 to 7 as a result of schistosomiasis. These observations suggest that there are schistosomiasis-associated changes of the catalytic and kinetic properties of hepatic ASB.

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References

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