Radiation Oncology Journal

The Korean Society for Radiation Oncology (대한방사선종양학회)
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The Protective Effects of Vitamin C on Hepatotoxicity Induced by Radiation

비타민 C의 방사선에 의한 간독성 완화 효과

  • Ahn Kijung (Department of Radiation Oncology, Pusan Paik Hospital, inje University) ;
  • Park Sungkwang (Department of Radiation Oncology, Pusan Paik Hospital, inje University) ;
  • Cho Heunglae (Department of Radiation Oncology, Pusan Paik Hospital, inje University) ;
  • Kang Kimun (Department of Radiation Oncology, Gyungsang National University) ;
  • Chung Duckwha (Department of Food Science and Technology, Gyungsang National University) ;
  • Kang Jinsoon (Department of Food Science, Jinju International University) ;
  • Chai Gyuyoung (Department of Radiation Oncology, Gyungsang National University)
  • 안기정 (인제대학교 의과대학 부산백병원 방사선종양학과) ;
  • 박성광 (인제대학교 의과대학 부산백병원 방사선종양학과) ;
  • 조흥래 (인제대학교 의과대학 부산백병원 방사선종양학과) ;
  • 강기문 (경상대학교 의과대학 방사선종양학교실) ;
  • 정덕화 (경상대학교 응용생명과학부) ;
  • 강진순 (진주국제대학교 식픔과학부) ;
  • 채규영 (경상대학교 의과대학 방사선종양학교실)
  • Published : 2004.12.01
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Abstract

Purpose: This study was carried out to determine the protective effects of vitamin C on the hepatotoxicily induced by radiation. Materials and Methods : The Spraque Dawley rats were randomly divided into 3 groups; the control group, the radiation exposed group, aud the radiation and vitamin C-treated group. SOD activity, ca-talase, malondialdehyde and liver enzymes were analyzed to assess the antioxidant effects of vitamin C. Results: The increased level of malondialdehyde and the decreased catalase activity that were induced by radiation were improved after vitamin C but there was no statistical significance among three groups. The superoxide dismutase activity of the liver was increased by vitamin C, but there were no statistically significant differences between the vitamin C-treated group and the non vitamin C-treated group. The level of liver enzymes in sera such as glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactate dehyrogenase and alkaline phosphatase were remarkably elevated by radiation. The levels of those enzymes were decreased in the vitamin C-treated group and statistical significance was noted for the GPT level ($p<0.01$). On the electromicrographic findings, the hepatic cell destruction was considerably decreased in the vitamin C-treated group. Conclusion: Vitamin C is thought to be an effective antioxidant against the hepatotoxicity induced by radiation.

목적: 본 연구는 방사선에 의한 간 손상에 대한 비타민 C의 보호 효과를 알아보기 위해서 시행되었다. 대상 및 방법: Sprague Dawley 실험 쥐를 대조군, 방사선 조사군, 방사선 조사 후 비타민 C 투여군, 3그룹으로 나누어 각 그룹에 무작위로 6마리를 분배하여 실험을 진행하였다. 비타민 C의 항산화 효과를 알아보기 위해서 Superoxide dismutase (SOD), 카탈라제, Malondialdehyde (MDA), 간 효소를 측정하고 그 값을 비교하였다. 결과: 방사선에 의해서 증가된 MDA수치와 감소된 SOD활성도 및 카탈라제의 활성도는 비타민 C투여로 개선되었으나 통계적 유의성은 없었다. GOT, GPT, LDH, ALP 수치들은 방사선 조사로 증가되었으나 비타민 C 투여로 감소하는 것을 관찰할 수 있었으며, GPT의 경우에는 통계적으로 유의하게 감소하였다. 전자 현미경 소견에서도 간세포 구조의 손상 정도가 비타민 C 투여군에서 뚜렷하게 감소하는 것을 관찰할 수 있었다. 결론: 비타민 C는 방사선에 의한 간세포 손상에 대해서 비록 통계적 유의성은 없었으나 항산화 효과를 보였다.

Keywords

References

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