Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Optimized Culture Conditions for Production of the chimaeric protein, Uropathogenic Escherichia coli Adhesin - Cholera Toxin A2B Subunits, in Escherichia coli TB1

  • Lee, Yong-Hwa (Division of Immunopharmacology, College of Pharmacy SungKyunKwan University) ;
  • Kim, Byung-Oh (Collgeg of Life Science and Natural Resources, Sangju National University) ;
  • Rhee, Dong-Kwon (Division of Immunopharmacology, College of Pharmacy SungKyunKwan University) ;
  • Pyo, Suh-Kneung (Division of Immunopharmacology, College of Pharmacy SungKyunKwan University)
  • Published : 2004.09.01
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Abstract

The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause for urinary tract infections. In our previous study, the Adhesin/CTXA2B was expressed as soluble recombinant chimaeric protein derived from the uropathogenic Escherichia coli adhesin genetically coupled to cholera toxin A2B (CTXA2B) subunit in Escherichia coli. Since it is very important to optimize IPTG concentration and culture temperature to maximize cell growth and productivity, These optimal culture factors were determined to increase the productivity of the expressed Adhesin/CTXA2B chimaeric protein in Escherichia coli TB1 carrying pMALfimH/ctxa2b. Our data demonstrate that optimal concentration of IPTG for increased production of chimaeric protein was 0.5 mM. Additionally, culture time was 10 hours and temperature, 37${\circ}C$.

Keywords

References

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