대한예방한의학회지 (Journal of Society of Preventive Korean Medicine)

대한예방한의학회 (Society of Preventive Korean Medicine)
권호 표지

삼백초 추출물의 항암활성과 카드뮴에 대한 독성억제효과

Effects of Saururus Chinensis Baill Extracts on Anticancer Activity and Cadmium Induced Cytotoxicity

  • 박승정 (원광대학교 대학원 한의학과) ;
  • 유현주 (원광대학교 한의학전문대학원 제3의학과) ;
  • 최희석 (원광대학교 한의학전문대학원 제3의학과) ;
  • 서병윤 (원광대학교 한의학전문대학원 제3의학과) ;
  • 양선호 (원광대학교 한의학전문대학원 제3의학과) ;
  • 김영훈 (원광대학교 대학원 한의학과) ;
  • 정재열 (원광대학교 한의학전문대학원 제3의학과) ;
  • 이기남 (원광대학교 한의학전문대학원 제3의학과)
  • Park Seung-Joung (Dept. of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Yoo Hyun-Ju (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi Hee-Seok (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Seo Byeong-Yun (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yang Seon-Ho (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Yeong-Hun (Dept. of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Jeong Jae-Yeal (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Ki-Nam (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2004.12.29
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초록

1. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus chinensis Baill. showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Baill. possessed a potential antitumorous agent. 2. In vitro the antitoxic activity of ethanol extract of Saururus Chinensis Bail on NIH 3T3 fibroblasts was evaluate by the MTT (3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl-2H-tetra캐lium bromide) and SRB (sulforhodamine B protein) assays. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that Saururus Chinensis Bail extract retains a potential antitoxic activity. 3. Complexation of Cd (II) ion with ligands such as quercetin has been determined by UV-vis spectrophotometric method in tris buffer solution at various pH. It was found that only 1 : 1 Cd-complex is formed by the interaction between catechol moiety in ring B and cadmium [Cd(II)] in aqueous solution. The spectral parameters for Cd-complex were determined by Beer's law at various pH. It has shown that 1 : 1 Cd-complex has a maximum absorbance and red shift by the alkaline pH.

키워드

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