Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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THE ROLE OF BONE MORPHOGENETIC PROTEIN IN THE TOOTH CULTURE

치아 기관배양시 골형성단백의 역할에 관한 연구

  • Chung, Il-Hyuk (Dept. of Oral and Maxillofacial Surgery, Craniofacial Tissue Engineering Laboratory of BK21 Human Life Science and the Intellectual Biointerface Engineering Center, College of Dentistry, Seoul National University) ;
  • Chung, Jong-Hoon (Dept. of Biosystems and Agricultural Engineering, Inst. of Ag. Sci. and Tech., Chonnam National University) ;
  • Choung, Pill-Hoon (Dept. of Oral and Maxillofacial Surgery, Craniofacial Tissue Engineering Laboratory of BK21 Human Life Science and the Intellectual Biointerface Engineering Center, College of Dentistry, Seoul National University)
  • 정일혁 (서울대학교치과대학 구강악안면외과학교실, 악안면기형 특수진료실, BK21 악안면기형재건 조직공학팀, 지능형생체계면공학센터) ;
  • 정종훈 (전남대학교 농업생명과학대학 생물산업공업과) ;
  • 정필훈 (서울대학교치과대학 구강악안면외과학교실, 악안면기형 특수진료실, BK21 악안면기형재건 조직공학팀, 지능형생체계면공학센터)
  • Published : 2004.10.30
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Abstract

Objectives : The proper development of the facial structures relies upon a sequence of tightly regulated signaling interactions between the ectoderm and mesoderm involving the participation of several families of signaling molecules. Among these, bone morphogenetic proteins (BMPs) have been suggested to be a key signal that regulates the development of the mandible and the initiation and morphogenesis of the teeth. The aim of this study was to examine the artificial development of the mandibular structures and to examine the role of BMPs on tooth morphogenesis and differentiation using an organ culture system. Materials and Methods : The tooth germs from Ed 11.5, 13.5 mice were dissected, and transplanted into the diastema of the mandible primordia. The mandibles containing the transplanted tooth germs were cultured in vitro. During this period, beads soaked with BMP4 were implanted around the transplanted tooth germs. In addition, a diastema block containing the transplanted tooth germ was dissected, then transferred to an adult mouse kidney. After the organ culture, the developing mandibular explant was removed from the kidney and prepared for the tissue specimens. Odontogeneis of the transplanted tooth germs was examined after Hematoxylin-eosin, Masson-trichrome staining. Results : Proliferation and differentiation of the tooth germs cultured in the diastema was observed. In the BMP4-treated tooth germs, the formation of the first and second molars was noted. The crown of the developing tooth showed the formation of a mature cusp with the deposition of enamel and dentin matrix. In conclusion, it was confirmed that BMP4 is involved in the formation of a dental crown and the differentiation of ameloblasts and odontoblasts of the molar tooth during the development of the transplanted tooth germs.

Keywords

References

  1. Chai Y, Jiang X, Ito Y, Bringas P, Han J, Rowitch DH, Soriano P, MaMahon AP, Sucov HM. Fate of the mammalian cranial neral crest during tooth and mandibular morphogenesis. Development 2000;127:1671-1679.
  2. Stottman RW, Anderson RM, Klingensmith J. The BMP antagonists chordin and noggin have essential but redundant roles in mouse mandibular outgrowth. Dev Biol 2001;240:457-473. https://doi.org/10.1006/dbio.2001.0479
  3. Thesleff I. Epithelial-mesenchymal signalling regulating tooth morphogenesis. J Cell Science 2003;116:1647-1648. https://doi.org/10.1242/jcs.00410
  4. Peters H, Balling R. Teeth where and how to make them. Trends Genet 1999;15:59-65. https://doi.org/10.1016/S0168-9525(98)01662-X
  5. Thesleff I. Genetic basis of tooth development and dental defects. Acta Odontol Scand 2000;58:191-194. https://doi.org/10.1080/000163500750051728
  6. Thesleff I, Nieminen P. Tooth morphogenesis and cell differentiation. Curr Opin Cell Biol 1996;8:844-850. https://doi.org/10.1016/S0955-0674(96)80086-X
  7. Corbourne MT, Sharpe PT: Tooth and jaw: molecular mechanism of patterning in the first branchial arch. Arch Oral Biol 2003;48:1-14. https://doi.org/10.1016/S0003-9969(02)00208-X
  8. Vainio S, Karavanova J, Jowetta A, Thesleff I. Identification of BMP-4 as a signal mediating secondary induction between epithelial and mesenchymal tissues during early tooth development. Cell 1993;75:45-58. https://doi.org/10.1016/S0092-8674(05)80083-2
  9. Francis-West PH, Tatla T, Brickell PM. Expression patterns of the bone morphogenetic protein genes Bmp-4 and Bmp-2 in the developing chick face suggest a role in outgrowth of the primordia. Dev Dyn 1994;201:168-178. https://doi.org/10.1002/aja.1002010207
  10. Aberg T, Wozney J, Thesleff I. Expression patterns of bone proteins (BMPs) in the developing mouse tooth suggest roles in morphogenesis and cell differentiation. Dev Dyn 1997;210:383-396. https://doi.org/10.1002/(SICI)1097-0177(199712)210:4<383::AID-AJA3>3.0.CO;2-C
  11. Helder MN, Karg H, Berovoets TJ, Vukicevic S, Burger EH, D'souza RN, Woltgens JH, Karsenty G, Bronckers AL. Bone morphogenetic protein-7 (osteogenic protein-1, OP-1) and tooth development. J Dent Res 1998;77:545554.
  12. Karavana I, Vainio S, Thesleff I. Transient and recurrent expression of the Egr-1 genein epithelial and mesenchymal cells during tooth morphogenesis suggests involvement in tissue interactions and in determination of cell fate. Mech Dev 1992;39:41-50. https://doi.org/10.1016/0925-4773(92)90024-E
  13. Jowett AK, Vainio S, Ferguson MW, Sharpe PT, Thesleff I. Epithelialmesenchymal interactions are required for msx-1 and msx-2 gene expression in the developing murine molar tooth. Development 1993;117:461-470.
  14. Mina M, Kollar EJ. The induction of odontogenesis in non dental mesenchyme combined with early murine mandibular arch epithelium. Arch Oral Biol 1987;32:123127.
  15. Bei M, Maas RL. FGFs and BMP4 induce both Msx1-independent and Msx1-dependent signaling pathways in early tooth development. Development 1998; 125:4325-4333.
  16. Bei M, Kratochwil, K, Maas RL. BMP4 rescues a non-cell-autonomous function of Msx1 in tooth development. Development 2000;127:4711-471.
  17. Trowell OA. The culture of mature organs in a synthetic medium. Exp Cell Res 1959;16:118-147. https://doi.org/10.1016/0014-4827(59)90201-0
  18. Ikeda T, Takahashi H, Suzuki A, Ueno N, Yokose S, Yamaguchi A, Yoshike S. Cloning of rat type I receptor c DNA for bone morphogenetic protein-2 and bone morphogenetic protein-4 and the localization compared with that of ligands. Dev Dyn 1996;206:318-329. https://doi.org/10.1002/(SICI)1097-0177(199607)206:3<318::AID-AJA9>3.0.CO;2-L
  19. Tucker AS, MatthewsKL, Sharpe PT. Transformation of tooth type induced by inhibition of BMP signaling. Science 1998;282:1136-1138. https://doi.org/10.1126/science.282.5391.1136
  20. Tabata MJ, Fujii T, Liu JG, Ohmori T, Abe M, Wakisaka S, Iwamoto M, Kurisu K. Bone morphogenetic protein 4 is involved in cusp formation in molar tooth germ of mice. Eur J Oral Sci 2002;110:114-120. https://doi.org/10.1034/j.1600-0722.2002.11194.x
  21. Jernvall J, Aberg T, Kearney S, Thesleff I. The life history of an embryonic signaling center: BMP-4 induced p21 and is associated with apoptosis in the mouse tooth enamel knot. Development 1998;125:161-169.
  22. Vaahtokari A, Aberg T, Jernvall J, Keranen S, Thesleff I. The enamel knot as a signaling center in the developing mouse tooth. Mech Dev 1996;54:39-43. https://doi.org/10.1016/0925-4773(95)00459-9
  23. Vaahtokari A, Aberg T, Thesleff I. Apoptosis in the developing tooth: association with an embryonic signaling center and suppression by EGF and FGF-4. Development 1996;122:121-129.
  24. Jernvall J, Keranen SV, Thesleff I. Evolutionary modification of development in mammalian teeth: quantifying gene expression patterns and topography. Proc Natl Acad Sci USA 2000;97:14444-14448. https://doi.org/10.1073/pnas.97.26.14444
  25. Jernvall J, Thesleff I. Reiterative signalling and patterning during mammalian tooth morphogenesis. Mech Dev 2000;92:19-29. https://doi.org/10.1016/S0925-4773(99)00322-6
  26. Takahashi H, Ikeda T. Transcripts for two members of the transforming growth factor-beta superfamily Bmp-3 and Bmp-7 are expressed in developing rat embryos. Dev Dyn 1996;207:439-449. https://doi.org/10.1002/(SICI)1097-0177(199612)207:4<439::AID-AJA8>3.0.CO;2-I
  27. Jernvall J, Kettunen P, Karavanova K, Martin LB, Thesleff I. Evidence for the role of the enamel knot as a control center in mammalian tooth cusp formation: non-dividing cells express growth stimulating Fgf-4 gene. Int J Dev Biol 1994;38:463-469.
  28. Begue-Kirn C, Smith AJ, Loriot M, Kupferle C, Ruch JV, Lesot H. Comparative analysis of TGF betas. BMPs, IGF1, msxa, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation. Int J Dev Biol 1994;38:405-420.
  29. Obara N. Expresssion of the neural cell adhesion molecule during mouse tooth development. Connect Tissue Res 2002;43:212-215. https://doi.org/10.1080/03008200290000781
  30. Peterka M, Vonesch JL, Ruch JV, Cam Y, Peterkova R, Lesot H. Position and growth of upper and lower tooth primordia in prenatal mouse-3D study. J Craniofac Genet Dev Biol 2000;20:35-43.
  31. Freshney RI. Culture of animal cells, a manual of basic technique (ed 4), Danveers, MA, Willey-Liss, 2000, pp 396-399.