Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Determination of polyphenolic compounds in grape seed extracts using reverse-phase high performance liquid chromatography

  • Wang, Chong-Zhi (Tang Center for Herbal Medicine Research, Department of Anesthesia & Critical Care, Pritzker School of Medicine, University of Chicago) ;
  • Osinski, Joachim (Tang Center for Herbal Medicine Research, Department of Anesthesia & Critical Care, Pritzker School of Medicine, University of Chicago) ;
  • Shao, Zuo-Hui (Tang Center for Herbal Medicine Research, Emergency Resuscitation Research Center, Pritzker School of Medicine, University of Chicago) ;
  • Basila, Daniel (Tang Center for Herbal Medicine Research, Department of Anesthesia & Critical Care, Pritzker School of Medicine, University of Chicago) ;
  • Kim, Stephen (Tang Center for Herbal Medicine Research, Department of Anesthesia & Critical Care, Pritzker School of Medicine, University of Chicago) ;
  • Yuan, Chun-Su (Tang Center for Herbal Medicine Research, Department of Anesthesia & Critical Care, Committee on Clinical Pharmacology, Pritzker School of Medicine, University of Chicago)
  • Published : 2004.12.30
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Abstract

Oxidative stress is associated with many kinds of chronic diseases. Antioxidants such as polyphenols are compounds that protect cells against the damaging effects of reactive oxygen species. Grape seeds are considered good resources of polyphenols, and grape seed extracts have a very strong antioxidant effect. In the present study, we established a simple gradient reverse-phase high performance liquid chromatography method to determine polyphenol content from three different grape seed resources. An ODS (2), $150\;{\times}\;3.2\;mm$ column has been employed, and six polyphenols have been determined: gallic acid, protochatechuic acid, (+)-catechin, (-)-epicatechin, procyanidin B2, and epicatechin gallate. Catechin and epicatechin were the main polyphenol compounds in all three extracts. The amount of procyanidin B2 was higher in Extract 1 (from a company of China), while Extract 2 (extracted in our lab) and Extract 3 (from a company of USA) contained higher proportions of epicatechin gallate. For the total polyphenol content, Extract 1 was much higher than that of Extract 2 and 3. The results suggest that the dietary dose of grape seed extracts from different resources should be adjusted according to polyphenol content.

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References

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