Advances in Traditional Medicine

  • 제4권3호
  • /
  • Pages.171-178
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  • 2004
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  • 2662-4052(pISSN)
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  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
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Ginseng radix induces apoptosis in HL-60 cells and its mechanism as little relation with TNF-α production

  • Koo, Hyun-Na (Department of Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Shin, Soon-Shik (Department of Prescriptionlogy, College of Oriental Medicine, Dongeui University) ;
  • Park, Jin-Han (College of Pharmacy, Wonkwang University) ;
  • Kim, Na-Hyung (College of Pharmacy, Wonkwang University) ;
  • Hong, Seung-Heon (College of Pharmacy, Wonkwang University) ;
  • Kim, Hyung-Min (Department of Pharmacology, College of Oriental Medicine, Kyung Hee University)
  • 발행 : 2004.09.30
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초록

Ginseng radix, the root of Panax ginseng C. A. Meyer (Araliaceae), is a medicinal plant used world-widely and has been reported to have various biological effects. To investigate the effects of Ginseng radix on HL-60 cell apoptosis, MTT assay, DNA fragmentation assay and flow cytometry were performed on HL-60 cells. Cells were treated with Ginseng radix at different concentrations $(10^{-4},\;10^{-3}\;and\;10^{-2};\;dilution\;rate)$. Ginseng radix significantly induced cells apoptosis with a time- and dose-dependent manner. To determine whether Ginseng radix-induced apoptosis is due to increase of tumor necrosis factor $(TNF-{\alpha})$ secretion, enzyme-linked immunosorbent assay was performed on HL-60 cells. Unexpectedly, Ginseng radix $(96\;{\pm}\;5\;pg/ml)$ significantly decreased the $TNF-{\alpha}$ secretion compared with control $(174\;{\pm}\;14\;pg/ml)$. Furthermore, Ginseng radix with $rIFN-{\gamma}$ synergistically increased nitric oxide production in mouse peritoneal macrophages. Taken together, our data indicate that Ginseng radix induce apoptosis on HL-60 cells without increase of $TNF-{\alpha}$ secretion and could be used for a supplementary remedy of cancer.

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참고문헌

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