Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Invitro antifilarial potential of the leaf extract of Oscimum sanctum on cattle filarial parasite Setaria cervi

  • Waseem, Rizvi (Department of Pharmacology, J. N. Medical College, Aligarh Muslim University) ;
  • K.C., Singhal (Department of Pharmacology, J. N. Medical College, Aligarh Muslim University) ;
  • Nakhat, Haider (Department of Pharmacology, J. N. Medical College, Aligarh Muslim University) ;
  • Anil, Kumar (Department of Pharmacology, J. N. Medical College, Aligarh Muslim University)
  • Published : 2004.03.30
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Abstract

The effect of aqueous and alcoholic extract of the leaves of Ocimum. sanctum was studied on the spontaneous movements of the whole worm (w.w) preparation and nerve muscle (n.m.) complex of Setaria cervi (S. cervi) and on the survival of microfilariae (m.f.) in vitro. Both the extracts caused inhibition of the spontaneous motility of the w.w. and n.m. complex of S. cervi characterized by initial stimulation followed by reversible paralysis, aqueous extract at a higher concentration showed immediate effect and irreversible paralysis. The concentration required to inhibit the movements of n.m. complex was $1/4^{th}$ for aqueous and $1/3^{rd}$ for alcoholic extract compared to that for the w.w., suggesting a cuticular permeability barrier. On the m.f. the lethal concentration (LC 50 and LC 90) were 35 and 50 ng/ml for aqueous whereas, 60 and 85 ng/ml for alcoholic extracts respectively.

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References

  1. Aubry NL, Cowell P, Davey MJ, Shevda S. (1970) Aspects of Pharmacology of a new anthelmintic: Pyrantel. Br. J. Pharmacol. 38, 332-344. https://doi.org/10.1111/j.1476-5381.1970.tb08521.x
  2. Chopra RN, Chopra IC, Handa KL, Kapoor LD. (1982) Indigenous drugs of India, pp. 517. Academic publishers, Calcutta-New Delhi.
  3. Christ D, Goebel M, Saz HJ. (1990) Actions of acetylcholine and GABA on spontaneous contractions of filarid Dipetalonema viteae. Br. J. Pharmacol. 101, 971-977. https://doi.org/10.1111/j.1476-5381.1990.tb14190.x
  4. Del Castillo J, De Mello WC, Morales T. (1963) The Physiological role of acetylcholine in the neuromuscular system of Ascaris lumbricoides. Arch. Int. Physiol Biochem. 71, 741-757. https://doi.org/10.3109/13813456309092194
  5. Devi PV, Ganasoundari A. (1995) Radio protective effect of leaf extract of Indian medicinal plants Ocimum sanctum. Indian J. Exp. Biol. 33, 205-208.
  6. Fetterer RH. (1986) Transcuticular solute movement in parasitic nematodes: relationship between non polar solute transport and partition coefficient. Comp. Biochem. Physio. 84A, 461-466.
  7. Jansen AM, Scheffer JJC, Ntezurubanza L, A Baerheim Svendsen. (1989) Antimicrobial activity of some ocimum species grown in Rwanda. J. Ethnophannacol. 26, 57-63. https://doi.org/10.1016/0378-8741(89)90113-X
  8. Kavindra Singh, Shalini Nagaich. (2002) Anthelmintic efficacy of the alcoholic extract of Ocimum sanctum against common poultry worms Ascaridia galli and Heterakis galliniae. J. Parasitic Diseases 26, 42-45.
  9. Martin BJ. (1982) Electrophysiological effects of piperazine and di ethylcarbamazine on Ascaris suum somatic muscle. Br. J. Pharmacol. 77, 255-265. https://doi.org/10.1111/j.1476-5381.1982.tb09294.x
  10. Savitri Godhwani, Godhwani JL, Vyas DS. (1987) An experimental study evaluating its anti-inflammatory, analgesic and antipyretic activity in animals. J. Ethnopharmacol. 21, 153-163. https://doi.org/10.1016/0378-8741(87)90125-5
  11. Saxena R, Jain S, Sharma RK. (1994) Nematicidal efficacy of leaf extract of Tulsi. Ocimum sanctum. Env. Appl. Bio. pp. 203-206.
  12. Singhal KC, Madan BR, Saxena PN. (1978) Effect of die ethylcarbamazine on Setaria cervi in vitro. Indian J. Physiol. Pharmacol. 22, 93-97.
  13. Singhal KC, Madan BR, Saxena PN. (1977) Effect of drugs on the nerve muscle complex of Setaria cervi. Indian J. Med. Res. 66, 517-521.
  14. Singhal KC, Madan BR, Saxena PN, Johri MBL. (1975) Effect of neurohormones and some other drugs on the movements of Setaria cervi Ind. J. Pharmacol. 7, 22-26.
  15. Singhal KC, Saxena PN, Johri MBL. (1973) Studies on the use of Setaria cervi for in vitro antifilarial screening. Jap. J. Pharmacol. 23, 793-797. https://doi.org/10.1254/jjp.23.793