Applied Biological Chemistry

  • 제47권4호
  • /
  • Pages.422-425
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  • 2004
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

식용 식물자원으로부터 활성물질의 탐색 -XIII. 개망초(Erigeron annuus L.) 꽃으로부터 triterpenoid의 분리-

Development of Biologically Active Compounds from Edible Plant Sources -XIII. Isolation of Triterpenoids from the Flower of Erigeron annuus L.-

  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정성제 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 방명호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 권병목 (한국생명공학연구원) ;
  • 김대근 (우석대학교 약학대학) ;
  • 박미현 ((주)이롬라이프) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Kim, Dong-Hyun (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Jung, Sung-Je (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Chung, In-Sik (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Park, Mi-Hyun (Erom Life Co. Ltd.) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 발행 : 2004.12.31
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초록

개망초의 꽃을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel, octadecylsilica gel(ODS) column chromatography 및 high performance liquid chromatography(HPLC)로 정제하여 3종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\alpha}-amyrenone(1),\;{\alpha}-amyrin(2)$ 및 ${\beta}-amyrin(3)$으로 동정하였다. 이들 3종의 triterpenoid는 개망초에서는 처음 분리되었다.

The flower of Erigeron annuus L. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three compounds were isolated through the repeated silica gel, ODS column and high performance liquid chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\alpha}-amyrenone,\;{\alpha}-amyrin\;and\;{\beta}-amyrin$. These three compounds were isolated for the first time from the flower of Erigeron annuus L.

키워드

참고문헌

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