Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources-XI -Analysis of Phytol Content in Several Varieties of Lactuca sativa L. by gas chromatography-

식용식물자원으로부터 활성물질의 탐색-XI -GC를 이용한 상추(Lactuca sativa L.)의 품종별 Phytol 함량 분석-

  • Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Lee, Jin-Hee (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Ji-Young (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Chung, Dae-Kyun (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Pai, Tong-Kun (Department of Food & Nutrition, Anyang University) ;
  • Kim, In-Ho (Korea Food Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University)
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이진희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 방면호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김지영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정대균 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 배동근 (안양대학교 식품영양학과) ;
  • 김인호 (한국식품개발연구원) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2004.12.31
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Abstract

An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.

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References

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