Development of Biologically Active Compounds from Edible Plant Sources-XI -Analysis of Phytol Content in Several Varieties of Lactuca sativa L. by gas chromatography-

식용식물자원으로부터 활성물질의 탐색-XI -GC를 이용한 상추(Lactuca sativa L.)의 품종별 Phytol 함량 분석-

  • Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Lee, Jin-Hee (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Ji-Young (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Chung, Dae-Kyun (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Pai, Tong-Kun (Department of Food & Nutrition, Anyang University) ;
  • Kim, In-Ho (Korea Food Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University)
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이진희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 방면호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김지영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 정대균 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 배동근 (안양대학교 식품영양학과) ;
  • 김인호 (한국식품개발연구원) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2004.12.31

Abstract

An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.

Keywords

References

  1. Bell, F. P. (1986) In Pharmacological control of Hyperlipidemia, Arterial cholesterol estenfication by acyl-CoA: cholesterol acyltransferase, its possible significance in atherogenesis and its inhibition by drugs. Science Publishers, pp. 409-422
  2. Heider, J. G. (1986) In Pharmacological control of Hyperlipidemia, Agent which inhibit cholesterol esterification in the intestine and their potential value in the treatment of hypercholesterolemia. Science Publishers, pp. 423-438
  3. Jung, E. B. and Shin, M. K. (1990) In Hyangyak-Daesajeon, Younglimsa, 7th edition, Seoul, Korea, p. 1060
  4. Jang, T. O., Bang, M. H., Song, M. C., Hong, Y. H., Kim, J. Y, Chung, D. K., Pai, T. K., Kwon, B. M., Kim, Y. K., Lee, H. S., Kim, I. H. and Baek, N.-I. (2003) Deveolopment of biologically active compounds from edible plant sources-V. Phytol, ACAT (Acyl-CoA;Cholesterol Acyltransferase) inhibitory diterpenoid from the leaves of Lactuca sativa L. J. Korean Soc. Agric. Chem. Biotechnol. 42, 267-270