Applied Biological Chemistry

  • 제47권2호
  • /
  • Pages.265-269
  • /
  • 2004
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

큰까치수영의 항산화 및 안지오텐신 전환 효소 저해 활성

Antioxidant and Inhibitory Activities on Angiotensin Converting Enzyme in Lysimachia clethroides Duby

  • 발행 : 2004.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

생리활성 보유 식물자원 발굴 및 이용을 위해 큰까치수영 지상부에 대한 항산화 및 항고혈압 활성을 검정하였으며 페놀화합물의 함량은 메탄올 추출물이 2.62%, 물 추출물이 0.23%였고 메탄올 추출물에서 얻어진 용매별 분획물 중에서는 에틸차세테이트 분획이 37.76%로 가장 함량이 높았다. Linoleic acid 자동산화에 대한 저해효과는 메탄올 추출물이 반응 6일째에 83%이상의 저해율을 보여 ${\alpha}-tocopherol$의 -2%보다 탁월하게 우수였으며 각 분획물 중에서는 핵산 및 에텔 분획이 매우 효과적으로 자동산화를 저해하였다. Superoxide anion 라디칼에 대헤서는 큰까치수영의 메탄올 추출물과 물 추출물이 $5{\sim}200\;{\mu}g/ml$의 농도에서 $86{\sim}109%$ 및 $96{\sim}122%$로 항산화제인 ascorbic acid의 $-4{\sim}69%$보다 월등하게 우수한 소거능을 보였으며 DPPH 라디칼에 대해서는 에틸아세테이트분획이 $26.8\;{\mu}g/ml$의 $RC_{50}$로 좋은 소거 효과를 나타내었다. ACE에 대한 저해활성은 $4,000\;{\mu}g/ml$의 농도에서 물 추출물(33%)보다는 메탄올 추출물(71%)이 효과적이었으며 분획물 중에서는 n-hexane fraction(133%), ether fraction(100%) 및 ethylacetate fraction(88%) 분획이 매우 효과적인 저해활성을 나타내었다.

This study was conducted to develop physiologically active plant materials from medicinal plants. Crude extracts and solvent fractions prepared from Lysimachia cletroides Duby were tested for their antioxidant and antihypertensive activities. For ellucidating antioxidant potential, inhibition rate on linoleic acid peroxidation, as well as scavenging activities on superoxide anion and 1,1-dipicrylphenylhydrazyl (DPPH) radical were evaluated. For analyzing antihypertensive effect, inhibitory activity on angiotensin converting enzyme (ACE) was done. Methanol extract of L. cletroides showed potent inhibition activity of 83% on linoleic acid peroxidation, which was more effective than -2% of ${\alpha}-tocopherol$ at $25\;{\mu}g/ml$. Methanol and water extracts exhibited strong scavenging activities of $86{\sim}109%$ and $96{\sim}122%$ on superoxide anion radical which was higher than $-4{\sim}69%$ of ascorbic acid at $5{\sim}200\;{\mu}g/ml$. Hexane, ether and ethylacetate fractions possessed 133, 100 and 88% inhibitory activities on ACE at $4,000\;{\mu}g/ml$, respectively. From the results, it was expected that Lysimachia cletroides could be a new antioxidant and antihypertensive resource.

키워드

참고문헌

  1. Evance, C. R., Halliwell, B. and Lunt, G. G. (1995) In Free radicals and oxidative stress: environment, drugs and food additives. Portland Press, pp. 1-31
  2. Sozmen, E. Y., Tanyakin, T., Onat, T., Kufay, F. and Erlacin, S. (1994) Ethanol-induced oxidative stress and membrane injury in rat erythrocytes. Eur. J. Clin. Chem. Clin. Biochem. 32, 741-744
  3. Frei, B. (1994) In Natural antioxidants in human health and disease. Academic Press, pp. 25-55
  4. Noh, H. and Song, K. B. (2001) Isolation of an angiotensin converting enzyme inhibitor from Oenanthe javanica. Agric. Chem. Biotechnol. 44, 98-99
  5. Oh, S. J., Kim, S. H., Kim, S. K., Baek, Y. J. and Cho, K. H. (1997) Angiotensin I-converting enzyme inhibitory activity of the K-casein fragments hydrolyzated by chymosin, pepsin, and trypsin. Korean J. Food Sci. Technol. 29, 1316-1318
  6. Shin, Z. I., Ahn, C. W., Nam, H. S., Lee, H. J., Lee, H. J, and Moon, T. H. (1995) Fractionation of angiotensin converting enzyme (ACE) inhibitory peptides from soybean paste. Korean J. Food Sci. Technol. 27, 230-234
  7. Park, D. C., Park, J. H., Gu, Y. S., Han, J. H., Byun, D. S., Kim, E. M., Kim, Y. M. and Kim, S. B. (2000) Effects of salted-fennented fish products and their alternatives on angiotensin converting enzyme inhibitory activity of Kimchi during fennentation. Food Sci. Biotechnol. 32, 920-927
  8. Cha, M. H. and Park, J. R .(2001) Isolation and characterization of the strain producing angiotensin converting enzyme inhibitor from soy sauce. J. Korean Soc. Food Sci. Nutr. 30, 594-599
  9. Ahn, S. W., Kim, Y. G., Kim, M. H., Lee, H. Y., and Seong,N. S. (1999) Comparison of biological activities on Rehmannia radix and R. radix preparata produced in Korea. Korean J. Medicinal Crop Sci. 7, 257-262
  10. An, B. J. and Lee, J. T. (1999) Isolation and characterization of angiotensin converting enzyme inhibitors from Camellia sinensis L. and their chemical structure determination. Food Sci. Biotechnol. 8, 285-289
  11. Yu, R., Park, S. A., Chung, D. K., Nam, H. S. and Shin, Z. I. (1996) Effect of soybean hydrolysate on hypertention in spontaneously hypertensive rats. J. Korean Soc. Food Sci. Nutr. 25, 1031-1036
  12. Bae, K. H. (2000) In Medicinal plants of Korea. Kyo-Hak Publishing Co., Seoul, Korea. p. 391
  13. Han, J. S. and Shin, D. H. (2001) Antimicrobial activity of Lysimachia clethroids Duby extracts on food-borne microorganisms. Korean J. Food Sci. Technol. 33, 774-783
  14. Lee, S. E., Seong, N. S., Park, C. G. and Seong, J. S. (2002) Screening for antioxidative activity of oriental medicinal plant materials. Korean J .Medicinal Crop Sci. 10, 171-176
  15. Nishikimi, N., Rao, N. A. and Yagi, K. (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun. 46, 849-854 https://doi.org/10.1016/S0006-291X(72)80218-3
  16. Cushman, D. W. and Cheung, H. S. (1973) Inhibition of homogenous angiotensin-converting enzyme of rabbit lung by synthetic venom peptides of Bothrops jararaka. Biochim. Biophys. Acta. 293, 453
  17. Kim, N. M., Sung, H. S. and Kim, W J. (1993) Effect of solvents and some extraction conditions on antioxidant activity in ciunamon extracts. Korean J .Food Sci. Technol. 25, 204-209
  18. Chen, J. H. and Ho, C. T. (1997) Antioxidant activities of caffeic acid and its related hydroxyciunarnic acid compounds. J. Agric. Food Chem. 45, 2374-2378 https://doi.org/10.1021/jf970055t
  19. Torel, J., Cillard, J. and Cillard, P. (1986) Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25, 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0
  20. Yoshida, T., Mori, K., Hatano, T., Okumura, T., Uehara, I., Komagoe, K., Fujita, Y. and Okuda, T. (1989) Studies on inhibition mechanism of autoxidation by tannins and flavonoids. V. Radical-scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 1919-1921 https://doi.org/10.1248/cpb.37.1919
  21. Chen, Y., Wang, M., Rosen, R .T. and Ho, C. T. (1999) 2,2-Diphenyl- 1-picrylhydrazyl radical-scavenging. active components from Polygonum multiflorum Thunb. J. Agric. Food Chem. 47, 2226-2228 https://doi.org/10.1021/jf990092f