Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Use of Accelerated Solvent Extraction Method for Determination of Residual Pesticides in Agricultural Products

농산물의 잔류농약분석을 위한 가속용매추출 (Accelerated Solvent Extraction) 방법의 이용

  • Lee, Sung-Woo (National Agricultural Products Quality Management service, Chonnam Branch office) ;
  • Seo, Hye-Young (Department of Food and Nutrition, Chosun University) ;
  • Han, Byung-Jae (Department of Food and Nutrition, Chosun University) ;
  • Jeong, Yang-Mo (Department of Food and Nutrition, Chosun University) ;
  • Kim, Jun-Hyoung (Department of Food and Nutrition, Chosun University) ;
  • No, Ki-Mi (Department of Food and Nutrition, Chosun University) ;
  • Kim, Kyong-Su (Department of Food and Nutrition, Chosun University)
  • 이성우 (국립농산물품질관리원전남지원) ;
  • 서혜영 (조선대학교 자연과학대학 식품영양학과) ;
  • 한병재 (조선대학교 자연과학대학 식품영양학과) ;
  • 정양모 (조선대학교 자연과학대학 식품영양학과) ;
  • 김준형 (조선대학교 자연과학대학 식품영양학과) ;
  • 노기미 (조선대학교 자연과학대학 식품영양학과) ;
  • 김경수 (조선대학교 자연과학대학 식품영양학과)
  • Published : 2004.06.30
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Abstract

ASE (accelerated solvent extraction) was used to develop an analytical method for reducing the sample preparation time in pesticide residues analyses of agricultural produce. The conditions of ASE were as follows: preheat 1 min, heat 5 min, static 1 min, solvent flush% 60 vol., nitrogen purge 60 sec, 4 cycles, 1,500 psi pressure, and $100^{\circ}C$ temperature. When n-hexane:acetone (4 : l, v/v) was used as, an extraction solvent, the extraction and purification efficiency of ASE did not show the considerable difference compared with solvent extraction, and the reduction of extraction solvent did not affect the performance of extraction. It was proved that ASE method can reduce, sample preparation time as the extraction and purification steps were combined, and the extraction solvent was significantly reduced.

농약성분 분석에 있어서 시료 전처리시간을 단축할 수 있는 분석방법을 개발하기 위해 농산물 중에 잔류하고 있는 잔류농약성분을 대상으로 ASE(accelerated solvent extraction) 방법을 활용한 자동화된 전처리 방법을 검토하였다. ASE 추출조건은 preheat 1 min, heat 5 min, static 1 min, solvent flush% 60 vol, nitrogen purge 60 sec, cycle 4, pressure 1500 psi, temperature $100^{\circ}C$를 선택하였다. 이 분석조건과 n-hexane:acetone(4 : 1, v/v)를 추출용매로 사용하였을 때, ASE 추출과 정제능력은 solvent extraction 방법과 유의적인 차이를 보이지 않았고, 추출 용매의 감소는 추출산물의 재현성에 큰 영향을 미치지 않았다. ASE 방법은 잔류농약 분석 시 소요되는 유기용매의 양을 감소하고 추출과 정제를 동시에 수행함으로써 동결건조 시간을 제외한 전처리 시간이 단축되어 분석비용 또한 solvent extraction에 비해 감소시킬 수 있었다.

Keywords

References

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