Development of Biologically Active Compounds from Edible Plant Sources-VIII. - Isolation of Platelet Aggregation Inhibitory Compounds from the Arils of Euphoria longana L. -

식용 식물자원으로부터 활성물질의 탐색-VIII. - 용안육(Euphoria longana L.)으로부터 분리된 uridine의 혈소판 응집 저해 효과 -

The arils of Euphoria longana L. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the n-BuOH fraction, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as 1,1-dimethyl-2propenyl $1-O-{\beta}-D-glucopyranoside$, ethyl ,${\beta}-D-glucopyranoside$, 5-(hydroxymethyl)-2-furfur-aldehyde and uridine. Uridine exhibited inhibition effect of 79% on platelet aggregation at the concentration of$5\;{\mu}g/ml$.

용안육을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 n-BuOH 분획을 silica gel, ODS 및 Sephadex LH-20 column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS및 IR 등의 스펙트럼 데이터를 해석하여, 1,1-dimethyl-2-propenyl $1-O-{\beta}-D-glucopyranoside$, ethyl ${\beta}-D-glucopyranoside$, 5-(hydroxymethyl)-2-furfuraldehyde 및 uridine으로 동정하였다. Uridine은 $5\;{\mu}g/ml$의 농도에서 collagen으로 유도한 혈소판 응집을 78% 저해하였다.