Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Selection and Identification of Auxin-Producing Plant Growth Promoting Rhizobacteria having Phytopathogen-antagonistic activity

Auxin과 항진균물질을 생산하는 식물생장촉진근권세균의 분리동정 및 특성

  • Kwon, Do-Hvung (Department of Natural Resource, Yeung Nam University) ;
  • Choi, Jun-Hyung (Department of Natural Resource, Yeung Nam University) ;
  • Jeung, Hee-Kyung (Department of Natural Resource, Yeung Nam University) ;
  • Lim, Jong-Hui (Department of Natural Resource, Yeung Nam University) ;
  • Joo, Gil-Jae (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Kim, Sang-Dal (Department of Natural Resource, Yeung Nam University)
  • 권도형 (영남대학교 자연자원대학 응용미생물학과) ;
  • 최준형 (영남대학교 자연자원대학 응용미생물학과) ;
  • 정희경 (영남대학교 자연자원대학 응용미생물학과) ;
  • 임종희 (영남대학교 자연자원대학 응용미생물학과) ;
  • 주길재 (경북대학교 농업과학기술연구소) ;
  • 김상달 (영남대학교 자연자원대학 응용미생물학과)
  • Published : 2004.03.31
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Abstract

This study was investigated the physiological properties of auxin-producing bacteria that have plant growth promoting activity and plant pathogen antagonistic ability. Auxin-producing bacteria were isolated from field soils of Gyeongsan, Korea. Selected strains were identified as a Pseudumonas fulva N21 and a Pantoea agglomerans; K35 by morphological and physiological test, and Biolog (Microlog) system. Auxins were determined by Salkowski in vitro test and mungbean adventitious root induction bioassay. Also produced indole-3-acetic acid (IAA) was identified by TLC. During cell growth, auxin production were highest in their idiophase after log phase and $35^{\circ}C$ at pH 7.5.

경작지 근권토양에서 식물생장촉진물질인 auxin라 항진균물질을 동시에 생산하는 농업용 미생물제제로 사용할 PGPR 균주를 선발하고 동정하였다. 경북 경산 일대의 다수확 밭토양의 근권토양에서 균주를 선발하고 그 중 auxin 생산성이 높은 2균주 K36, N21를 분리하였다. 이들 균주를 동정한 결과 K36은 Pantoea agglomerans이고 N21은 Pseudomonans fulva로 동정되었다. 선발된 균주의 식물생장촉진능을 in vivo 녹두발아촉진검사법으로 확인하였으며, 식물병원진균에 대한 길항력을 확인하였다. 배양조건에 따른 auxin 생산성을 조사한 결과 K36 균주는 $pH\;7.5,\;35^{\circ}C$, 37시간에서 최대로 나타났고, N21균주는 $pH\;7.5,\;35^{\circ}C$, 20시간에서 최대값을 나타내었다.

Keywords

References

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