Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Active Principles of the Methanol Extract of Korean Mistletoe Responsible for the Inhibitory Effect on the Proliferation of Human Tumor Cell Lines

한국산 겨우살이 전초의 Methanol 추출물로부터 암세포증식 저해성분의 분리

  • Published : 2004.06.30
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Abstract

A bioassay-guided fractionation of the whole extract of Viscum album var. coloratum Ohwi (Loranthaceae) led to the isolation of two triterpenoidal components; oleanolic acid (1) and ${\beta}-amyrin$ acetate (2), and a flavonoid, homoflavoyadorinin B (3) as well as large quantity of free fatty acid mixtures as active ingredients of the extract responsible for the antitumoral property. The EtOAc soluble fraction and BuOH soluble fraction of the extract demonstrated a significant inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system), and HCT-15 (colon) in vitro, whereas the remaining water soluble fraction exhibited a poor inhibition. The intensive phytochemical investigation of the EtOAc soluble fraction and BuOH soluble fraction of the extract indicated that the oleanolic acid (1) and large amounts of free fatty acid mixtures might be attributed to the in vitro antitumoral property of the whole extract of Viscum album var. coloratum.

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References

  1. Hajto, T., Berki, T., Boldizsar, E, and Nemeth, P (2003) Galactoside-specific plant lectin, Vzscum album agglutinin-I induces enhanced proliferation and apoptosis of murine thymocytes in vivo. Immunol. Lett. 86(1): 23-27 https://doi.org/10.1016/S0165-2478(02)00265-1
  2. Siegle, I., Fritz, P, McClellan, M., Gutzeit, S., and Murdter, T. E. (2001) Combined cytotoxic action of Vzscum album agglutinin-1 and anticancer agents against human A549 lung cancer cells. Anticancer Res. 21(4A): 2687-2691
  3. Kim, Y. K, Kim, Y S., Choi, S. U., and Ryu, S. Y. (2004) Isolation of flavonol rhamnosides from Loranthus tanakae and cytotoxic effect of them on human tumor cell lines. Arch. Pharm. Res. 27(1): 44-47 https://doi.org/10.1007/BF02980044
  4. Karagoz, A, Onay, E., Arda, N., and Kuru, A (2003) Antiviral potency of mistletoe (Viscum album ssp. album) extracts against human parainfluenza virus type 2 in Vero cells. Phytother. Res. 17(5): 560-562 https://doi.org/10.1002/ptr.1163
  5. Fernandez, T., Cerda, Z. P, Aulicino, P, Caldas, L. E., Wagner, M., Ricco, R., Hajos, S., Gurni, A, and Alvarez, E. (2003) Immunobiological features of the galactoside lectin L-Lc isolated from the Argentine mistletoe Ligaria cuneifolia. J. Ethnopharmacol. 85(1): 81-92 https://doi.org/10.1016/S0378-8741(02)00361-6
  6. Boneberg, E. M. and Hartung T. (2001) Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide- stimulated whole blood via inhibition of interleukin-10 production. J. Pharmacol. Exp. Ther. 298(3): 996-1000
  7. Tabiasco, J., Pont, E, Fournie, J. J., and Vercellone A (2002) Mistletoe viscotoxins increase natural killer cell-mediated cytotoxicity. Eur. J. Biochem. 269(10): 2591-2600 https://doi.org/10.1046/j.1432-1033.2002.02932.x
  8. Mengs, U., Gothel, D., and Leng-Pescchlow E. (2002) Mistletoe extracts standardized to mistletoe lectins in oncology: review on current status of preclinical research. Anticancer Res. 22(3): 1399-407
  9. Skehan, P, Streng, R., Scudiero, D., Monks, A, McMahon, J., and Vistica, D. (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer. Inst. 82: 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  10. Gaydou, E. M., Faure, R., and Wollenweber (1996) $\beta$-amyrin acetate epoxide from Canarina canariensis. Phytochemistry 42: 1115-1118 https://doi.org/10.1016/0031-9422(96)00118-5
  11. Balanehru, S. and Nagarajan, B. (1991) Protective effect of olenolic acid and ursolic acid against lipid peroxidation. Biochemistry International 24: 981-990
  12. Harada, H., Yamashita, U., Kurihara, H., Fukushi, E., Kawabata, J., and Kamei, Y. (2002) Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 22: 2587-2590
  13. Nano, J. L., Nobili, C., Girard-Pipau, F, and Rampal, P (2003) Effects of fatty acids on the growth of Caco-2 cells. Prostaglandins Leulwt Essent Fatty Acids. 69: 207-215 https://doi.org/10.1016/S0952-3278(03)00083-8
  14. Fukunaga, T., Kajikawa, I., Nishiya, K, Takeya, K, and Itogawa, H. (1989) Studies of the Japanese Mistletoe, Vzscum album L. var. coloratum Ohwi grown on different Host trees. Chem. Pharm. Bull. 37(5): 1300-1303 https://doi.org/10.1248/cpb.37.1300