High-Performance Liquid Chromatographic Method for Quantitative Estimation of an Antioxidant Principle Chlorogenic Acid in Saussarea costus and Arctium lappa

  • Published : 2004.02.28

Abstract

A simple quantitative HPLC method has been developed for differentiating two plants of Asteraceae family viz. S. costus and A. lappa by using a pharmacologically active constituent chlorogenic acid and symmetry C18 column clubbed with a binary gradient using acetonitrile: 0.1% phosphoric acid and detected using a PDA at 327 nm. Quantitatively chlorogenic acid was found to be more in A. lappa (0.140%) than in S. costus (0.087%).

Keywords

References

  1. Bergeron, C., Liversey, J. R, Awang, D. V. C., Amason, J. T., Rana, J., Baum, B. R. and Letchamo, W., A quantitatidve HPLC method for the quality assurance of Echinacea products on the North American market. Phytochemical Anal. 11, 207-215 (2000) https://doi.org/10.1002/1099-1565(200007/08)11:4<207::AID-PCA519>3.0.CO;2-T
  2. Chen, H. C., Chou, C. K., Lee, S. D., Wang, J. C. and Yeh, S. F, Active compounds from Saussurea lappa that suppress hepatitis B virus surface antigen gene expression in human hepatoma cells. Antiviral Res. 27, 99-109 (1995) https://doi.org/10.1016/0166-3542(94)00083-K
  3. Chhabra, B. R., Gupta, S., Jain, M. and Kalsi, P. S., Sesquiterpene lactones from Saussurea lappa. Phytochemistry. 49, 3801-804 (1998)
  4. Cho, J. Y, Baik, K. U, Jung, J. H. and Park, M. H., In vitro anti-inflammatory effect of cynaropicrin, a sesquiterpene lactone, from Saussurea Iappa. Eur. J. Pharmacol. 398, 399-407 (2000) https://doi.org/10.1016/S0014-2999(00)00337-X
  5. Dhillon, R. S., Kalsi, P. S., Singh, W. R, Gautam, V. K. and Chhabra, B. R., Guanolide from Saussurea lappa. Phytochemistry. 26, 41209-41210 (1987)
  6. Govindarajan, R., Vijayakumar, M., Rawat, A. K. S. and Mehrotra, S., Free radical scavenging activity of Picrorhiza kurrooa Indian J. Exp. Biol. 41, 875-879 (2003)
  7. Halliwell, B. and Gutteridge, J. M. C., Free radical in biology and medicine, 3rd eds, Oxford, New York, 1999, pp. 615-617
  8. Kalsi, P. S., Kaur, G., Sharma, S. and Talwar, K. K., Dehydrocostuslactone and plant growth activity of derived guaianolides. Phytochemistry. 23, 122855-122862 (1984)
  9. Lin, C. C., Lu, J. M., Yang, J. J., Chuang, S. C. and Ujiie, T., Antiinflammatory and radical scavenge effects of Arctium lappa. Am. J. Chinese. Med. 24, 127 137 (2000) https://doi.org/10.1142/S0192415X96000177
  10. Lin, S. C., Chyung, T. C, Lin, C. C., Veng, T. H., Lin, Y. H., Lin, S. Y. and Wang, L. Y, Hepatoprotective effects of Arctium lappa on carbontetrachloride and acetaminophen induced liver damage. Am. J. Chinese. Med. 28, 163-173 (2000) https://doi.org/10.1142/S0192415X00000210
  11. Shafiee, M., Carbonnart, M. A., Huart, J. B., Descomps, B. and Heger, C. L., Synergistic antioxidative properties of phenolics from natural origin towards low-density lipoproteins depend on oxidation system. J. Med. food. 5, 69-78 (2002) https://doi.org/10.1089/109662002760178140
  12. Singh, I. R, Talwar, K. K., Arora, J. K., Chhabra, B. R. and Kalsi, P. S., A biologically active guanolide from Saussurea lappa. Phytochemistry. 31, 2529-2531 (1992) https://doi.org/10.1016/0031-9422(92)83317-R
  13. Talwar, K. K., Singh, I. P. and Kalsi, P. S., A sesquiterpenoid with plant growth regulatory activity from Saussurea lappa. Phytochemistry. 31, 336-338 (1992) https://doi.org/10.1016/0031-9422(91)83069-W
  14. Taniguchi, M., Kataoka, T, Suzuki, H., Uramoto, M., Araok, M. J., Nishimura, T., Otake, N. and Nagai, K., Costunolide and dehydrocostus lactone as inhibitor of killing function of cytotoxic T. lymphocytes. Biosci. Biotechnol. Biochem. 59, 2064-2067 (1995) https://doi.org/10.1271/bbb.59.2064
  15. Washino, T., Kobayashi, H. and IKsawa, Y, Stmcture of lappaphen-a and lappaphen-b, new guainolides linked with a sulfur containing acetylenic compound from Arctium lappa. Agri. Biot. Chem. 51, 1475-1480 (1987) https://doi.org/10.1271/bbb1961.51.1475
  16. Watt, G., A Dictionary of the Economic products of India, Vol. II, Penodical Export, Delhi. 1890, PP.315
  17. Yamahara, J., Kobayashi, M., Miki, K., Kozuka, M., Sawada,T. and Fujimura, H., Cholatotic and antiulcer effect of Saussurea Radix and its components. Chem. Pharm. Bull. 33, 1285-1288 (1985) https://doi.org/10.1248/cpb.33.1285