대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)
- 제18권1호
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- Pages.27-48
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- 2004
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- 1226-8038(pISSN)
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- 2287-9463(eISSN)
황기건중탕(黃芪建中湯)이 Methotrexate로 유도된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)
The Effect of Hwanggigunjung-tang on Imunosuppression induced by Methotrexate in Rat
- Baek, Jung-Han (Department of Pediatrics, Gumi Oriental Medicine Hospital, Daegu Hanny University) ;
- Koo, Jin-Suk (Department of Pediatrics, Gumi Oriental Medicine Hospital, Daegu Hanny University)
- 발행 : 2004.08.31
초록
Objective: The purpose of this study was to investigate the effect of Hwanggigunjungtang(HGT) on white rats with deteriorated immunity caused by methotrexate. Methods: First, methotrexate was fed to the white rats once a day for 4 days. After the immune responses of the rats are deteriorated, dried extracts of HGT mixed in water was fed to the rats once a day for 14 days. And then we measured the percentage of B-cell and T-cell in peripheral blood, the percentage of CD3+CD4+ T-cell and CD3+CD8+ T-cell of blood sampled from spleen and peripheral region. Results: The percentage of B-cell of peripheral blood was not different statistically. The percentage of T-cell of peripheral blood was increased significantly in HGT group as compared with control group. The percentage of CD3+CD4+ T-cell of peripheral blood was increased significantly in HGT group as compared with control group. The percentage of CD3+CD8+ T-cell of peripheral blood was decreased in HGT group as compared with control group. The percentage of CD3+CD4+ T-cell of spleen was increased significantly in HGT group as compared with control group. The percentage of CD3+CD8+ T-cell of spleen was not different statistically. Conclusion: According to the above results, HGT has an effect of increasing immune responses on white rats with deteriorated immunity caused by methotrexate.