The Effect of Hwanggigunjung-tang on Imunosuppression induced by Methotrexate in Rat

황기건중탕(黃芪建中湯)이 Methotrexate로 유도된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

  • Baek, Jung-Han (Department of Pediatrics, Gumi Oriental Medicine Hospital, Daegu Hanny University) ;
  • Koo, Jin-Suk (Department of Pediatrics, Gumi Oriental Medicine Hospital, Daegu Hanny University)
  • 백정한 (대구한의대학교 부속 구미한방병원 소아과) ;
  • 구진숙 (대구한의대학교 부속 구미한방병원 소아과)
  • Published : 2004.08.31

Abstract

Objective: The purpose of this study was to investigate the effect of Hwanggigunjungtang(HGT) on white rats with deteriorated immunity caused by methotrexate. Methods: First, methotrexate was fed to the white rats once a day for 4 days. After the immune responses of the rats are deteriorated, dried extracts of HGT mixed in water was fed to the rats once a day for 14 days. And then we measured the percentage of B-cell and T-cell in peripheral blood, the percentage of CD3+CD4+ T-cell and CD3+CD8+ T-cell of blood sampled from spleen and peripheral region. Results: The percentage of B-cell of peripheral blood was not different statistically. The percentage of T-cell of peripheral blood was increased significantly in HGT group as compared with control group. The percentage of CD3+CD4+ T-cell of peripheral blood was increased significantly in HGT group as compared with control group. The percentage of CD3+CD8+ T-cell of peripheral blood was decreased in HGT group as compared with control group. The percentage of CD3+CD4+ T-cell of spleen was increased significantly in HGT group as compared with control group. The percentage of CD3+CD8+ T-cell of spleen was not different statistically. Conclusion: According to the above results, HGT has an effect of increasing immune responses on white rats with deteriorated immunity caused by methotrexate.

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