Effect of Hwang-Ryeon-Hae-Dok-Tang on the Release of IL-8 in Human Nasal Mucosal Fibroblast

黃連解毒湯의 사람 비점막 섬유아세포 IL-8 분비에 대한 효과

It is proposed that Hwang-Ryeon-Hae-Dok-Tang may modulate the immune response on allergy or asthma. Human nasal mucosal fibroblasts are a rich source cytokines, inflammatory mediators, and chemokines. Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. Objectives : The objective of this study was to investigate the effect of Hwang-Ryeon-Hae-Dok-Tang(HH) on the release of the IL-8 chemokine in human nasal mucosal fibroblasts after stimulation with cytokines like interleukin-4(IL-4), tumor necrosis factor- (TNF- ), interferon- (lFN- ), and interle ukin-l (IL-I ). Methods : To detect the release of IL-8, enzyme-linked immunosorbent assay(ELISA) kit was performed. The cytotoxicity was measured by MTT assay. Results : HH significantly inhibited the secretion of IL-8 with a dose-dependant manner. The effective dosage did not have the cytotoxicity on human nasal mucosal fibroblasts Conclusions : Results of our study show that HH would play an important role in modulation of IL-8 in human nasal mucosal fibroblasts.