Thermal Design of PCR Chip for LOC

랩온어칩을 위한 중합효소 연쇄반응 칩의 열설계

  • 김덕종 (한국기계연구원 열유체공정기술연구부 생물공정기계그룹) ;
  • 김재윤 (한국기계연구원 열유체공정기술연구부 생물공정기계그룹) ;
  • 박상진 (한국기계연구원 열유체공정기술연구부 생물공정기계그룹) ;
  • 허필우 (한국기계연구원 열유체공정기술연구부 생물공정기계그룹) ;
  • 윤의수 (한국기계연구원 열유체공정기술연구부 생물공정기계그룹)
  • Published : 2003.12.30

Abstract

In this work, thermal design of a PCR chip for LOC is systematically conducted. From the numerical simulation of a PCR chip based on the finite volume method, how to control the average temperature of a PCR chip and the temperature difference between the denaturation zone and the annealing zone is presented. The average temperature is shown to be controlled by adjusting heat input and a cooler as well as a heater is shown to be necessary to obtain three individual temperature zones for polymerase chain reaction. To reduce the time required, a heat sink for the cooler is not included in the calculation domain for the PCR chip and heat sink design is conducted separately by using a compact modeling method, the porous medium approach.

Keywords