Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
권호 표지

Molecular Biologic Analysis of c-kit Gene in Salivary Gland Carcinoma

타액선암에서 c-kit 유전자에 대한 분자생물학적 연구

  • Seo Kyu-Hwan (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Jung Kwang-Yoon (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Woo Jung-Soo (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Baek Seung-Kuk (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Choi Sung-Bae (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Kim Sang-Hee (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Kim In-Sun (Department of Pathology, Korea University, College of Medicine) ;
  • Kwon Soon-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
  • 서규환 (고려대학교 의과대학 이비인후과학교실) ;
  • 정광윤 (고려대학교 의과대학 이비인후과학교실) ;
  • 우정수 (고려대학교 의과대학 이비인후과학교실) ;
  • 백승국 (고려대학교 의과대학 이비인후과학교실) ;
  • 최성배 (고려대학교 의과대학 이비인후과학교실) ;
  • 김상희 (고려대학교 의과대학 이비인후과학교실) ;
  • 김인선 (고려대학교 의과대학 해부병리과학교실) ;
  • 권순영 (고려대학교 의과대학 이비인후과학교실)
  • Published : 2003.11.01
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Abstract

Objectives: The c-kit gene encodes a transmembrane receptor-type tyrosine kinase, which is known to have a significant role in the normal migration and development of germ cells and melanocytes. In the previous studies of c-kit gene, c-kit expressions showed only in adenoid cystic carcinomas, lymphoepithelioma-like carcinomas and myoepithelial carcinomas, but not in others and mutation was not found in any types of salivary carcinoma. We investigate the c-kit expression which may be useful to differentiating adenoid cystic carcinomas from others, and mutation of the gene which may not be exist nor the mechanism of c-kit activation in salivary carcinomas. Material and Methods: The archival tissue samples from 42 salivary carcinomas of major and minor salivary glands were studied for c-kit expression by immunohistochemistry and gene mutation by polymerase chain reaction amplification and single strand conformational polymorphism. Results: The c-kit expressions were noted in 22/24 adenoid cystic carcinomas, 7/9 mucoepidermoid carcinomas, 2/3 acinic cell carcinomas, 3/4 malignant mixed tumors, and one undifferentiated carcinoma. The mutation of c-kit gene was found in 3/24 adenoid cystic carcinomas, 3/8 mucoepidermoid carcinomas, one acinic cell carcinoma, and 2/4 malignant mixed tumors. Conclusion: c-kit protein overexpression is seen in a variety of salivary gland carcinomas, and the mutation of the gene may be the mechanism of c-kit activation in these neoplasms.

Keywords

References

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