IMMUNE NETWORK
- 제3권1호
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- Pages.47-52
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- 2003
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
생체 외 제대혈 배양에서 거대핵세포 조혈에 대한 Interleukin-11 (IL-11)의 효과
In Vitro Effect of Interleukin-11 (IL-11) on Megakaryopoiesis from Umbilical Cord Blood Cells
- 이국경 (순천향대학교 의과대학 내과학교실) ;
- 김찬규 (순천향대학교 의과대학 내과학교실) ;
- 이남수 (순천향대학교 의과대학 내과학교실) ;
- 김숙자 (순천향대학교 의과대학 임상분자생물학연구소) ;
- 정희정 (순천향대학교 의과대학 임상분자생물학연구소) ;
- 이규택 (순천향대학교 의과대학 내과학교실) ;
- 박성규 (순천향대학교 의과대학 내과학교실) ;
- 백승호 (순천향대학교 의과대학 내과학교실) ;
- 원종호 (순천향대학교 의과대학 내과학교실) ;
- 홍대식 (순천향대학교 의과대학 내과학교실) ;
- 박희숙 (순천향대학교 의과대학 내과학교실)
- Lee, Kuk-Kyung (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Kim, Chan-Kyu (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Lee, Nam-Su (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Kim, Sook-Ja (Institute for Clinical Biology Research, SoonChunHyang University College of Medicine) ;
- Cheong, Hee-Jeong (Institute for Clinical Biology Research, SoonChunHyang University College of Medicine) ;
- Lee, Kyu-Tack (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Park, Sung-Kyu (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Baick, Seung-Ho (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Won, Jong-Ho (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Hong, Dae-Sik (Department of Internal Medicine, SoonChunHyang University College of Medicine) ;
- Park, Hee-Sook (Department of Internal Medicine, SoonChunHyang University College of Medicine)
- 발행 : 2003.03.30
초록
Background: The megakaryopoiesis and platelet production is regulated by several hematopoietc factors such as thrombopoietin (TPO), interleukin-11 (IL-11) and interleukin- 3 (IL-3). IL-11 is a potent stimulator of megakaryopoiesis in vivo, and acts primarily as a megakaryocyte maturation factor in vitro and it can act synergistically with IL-3 and TPO. We performed this study to investigate the effects of recombinant human IL-11 (rhIL-11) with other hematopoietic factors on megakaryocyte colony formation in vitro. Methods: CD34+ cells were separated from umbilical cord blood and megakaryocyte colonies using MegaCult Assay Kit were cultured with rhIL-11, recombinant human IL-3 (rhIL-3), and recombinant human TPO (rhTPO) for 7 and 14 days. The number and percentage of CD34+ and CD41a+ cells were determined by flowcytometry. Results: The number of CD41a+ cells were