IMMUNE NETWORK

  • 제3권1호
  • /
  • Pages.23-28
  • /
  • 2003
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  • 1598-2629(pISSN)
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  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
권호 표지

B형 간염 바이러스의 X단백질에 대한 특이항체의 세포 내 발현

Expression of Intracellular Single Chain Antibody Specific to Hepatitis B Virus X Protein

  • 진영희 (아주대학교 의과대학 미생물학교실) ;
  • 김형일 (아주대학교 의과대학 미생물학교실) ;
  • 박선 (아주대학교 의과대학 미생물학교실)
  • Jin, Young Hee (Department of Microbiology, Ajou University School of Medicine) ;
  • Kim, Hyung-il (Department of Microbiology, Ajou University School of Medicine) ;
  • Park, Sun (Department of Microbiology, Ajou University School of Medicine)
  • Published : 2003.03.30
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Abstract

Background: Intracellular antibody specific to hepatitis B virus X protein (HBx) might be useful for studying the role of HBx in hepatocellular carcinogenesis and HBV replication. Methods: With variable region genes for H7 monoclonal anti-HBx Ab, we constructed a vector for bacterial expression of single chain Ab (scFv) and a vector for eukaryotic cell expression of it. The expression of H7 scFv and its binding activity against HBx was examined by immunoblotting and immunofluorescence microscopy. Results: H7 scFv expressed in bacterial cells retained reactivity to HBx. We demonstrated its intracytoplasmic expression in CosM6 eukaryotic cells. Conclusion: This is the first study showing the expression of intracellular anti-HBx Ab in eukaryotic cells. H7 scFv may be a good tool to study the function of HBx in HBV infection.

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