IMMUNE NETWORK
- 제3권1호
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- Pages.23-28
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- 2003
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
B형 간염 바이러스의 X단백질에 대한 특이항체의 세포 내 발현
Expression of Intracellular Single Chain Antibody Specific to Hepatitis B Virus X Protein
- Jin, Young Hee (Department of Microbiology, Ajou University School of Medicine) ;
- Kim, Hyung-il (Department of Microbiology, Ajou University School of Medicine) ;
- Park, Sun (Department of Microbiology, Ajou University School of Medicine)
- 발행 : 2003.03.30
초록
Background: Intracellular antibody specific to hepatitis B virus X protein (HBx) might be useful for studying the role of HBx in hepatocellular carcinogenesis and HBV replication. Methods: With variable region genes for H7 monoclonal anti-HBx Ab, we constructed a vector for bacterial expression of single chain Ab (scFv) and a vector for eukaryotic cell expression of it. The expression of H7 scFv and its binding activity against HBx was examined by immunoblotting and immunofluorescence microscopy. Results: H7 scFv expressed in bacterial cells retained reactivity to HBx. We demonstrated its intracytoplasmic expression in CosM6 eukaryotic cells. Conclusion: This is the first study showing the expression of intracellular anti-HBx Ab in eukaryotic cells. H7 scFv may be a good tool to study the function of HBx in HBV infection.