Journal of Acupuncture Research
- 제20권5호
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- Pages.50-62
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- 2003
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
봉독약침액(蜂毒藥鍼液)이 비만세포주의 CD/cytokine 유전자(遺傳子) 발현(發現)에 미치는 영향(影響)
Microarray Analysis of CD/cytokine Gene Expression in Human Mast Cell treated with Bee Venom
- Lee, Woong-kyung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
- Kang, Sung-keel (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
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Koh, Hyung-kyun
(Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University)
- 투고 : 2003.08.25
- 심사 : 2003.09.20
- 발행 : 2003.10.20
초록
Objective: Bee Venom(BV) has been used for various kinds of inflammatory or painful conditions in Oriental Medicine clinics, and there publishes reports on its therapeutic effects and the probable mechanism of those therapeutic effects, where CDs and cytokines plays important role. This study investigated the influences of bee venom on the expressions of CDs and cytokines of HMC cell line Methods: In this study we analysed the expression profile of HMC cell line treated with BV of 10-2ug/ml in relation to that of HMC cell line treated with vehicle by way of CD/cytokine microarray hybridization with 342 genes on it. Results: There were no upregulated genes by more than 3 fold, while there showed some downregulated genes by less than 1/3 fold as follows: colony stimulating factor 2, CD122, IL-7, CD112, TNF-alpha, CD138, CD166, TGFbetaR2, CD42b, CD62L, CD111, interleukin 10 receptor alpha, colony stimulating factor 1(macrophage), CD38 antigen(p45), CD121a, CD33 antigen(gp67), colony stimulating factor 1 receptor, B cell linker protein (SLP65) mRNA, CD94, alanyl(membrane) aminopeptidase, immunoglobulin(CD79A) binding protein 1, CD205, CD241, CD207, CDw121b, integrin alpha L(CD11a), integrin beta 1(CD29), CD91, CD42b. Conclusions: Bee venom treatment induced downregulation of some CDs or cytokines including