Screening for Biological Activity of Crude Extracts from Medicinal plants

생약추출물로부터 생리활성의 검색

The biological effects of the crude extracts from medicinal plants, Brachyglottis monroi and Trichocolea hatcheri were investigated. The crude ethanol extract inhibited the growth of the Gram positive bacterium Bacillus subtilis (ATCC 19659, 1 mm inhibition zone at $150{\mu}g/disc$) and the dermatophyte Trichophyton mentagrophytes (ATCC 28185, 6 mm inhibition zone at $150{\mu}g/disc$), and was toxic to P388 murine leukaemia cells ($IC_{50}$ $23.96{\mu}g/mL$ at $75{\mu}g/disc$). B. monroi ethanol extract showed stronger antiviral activity than that of T. hatcheri against Herpes simplex Type I virus (ATCC VR 733) and Polio Type I virus (Pfizer vaccine strain) (50% activity, @ 5 mg/ml at $150{\mu}g/disc$). The crude ethanol extract of T. hatcheri showed stronger antimicrobial activity than that of B. monroi. However, this extract was inactive against P388 murine leukaemia cells.

뉴질랜드의 자생식물인 Brachyglottis monroi와 Trichocolea hatcheri의 에탄올추출물에 대한 생리활성을 조사한 결과, 그람음성균인 Bacillus subtilis (ATCC 19659, 1 mm inhibition zone at $150{\mu}g/disc$)와 피부곰팡이균인 Trichophyton mentagrophytes (ATCC 28185, 6 mm inhibition zone at $150{\mu}g/disc$)에 대한 억제효과가 관찰되었으며, 이들 추출물은 P388 murine leukaemia cells ($IC_{50}$ $23.96{\mu}g/mL$)에서도 세포독성이 나타났다. B. monroi의 에탄올추출물의 항바이러스효과는 T. hatcheri의 에탄올추출물보다 강한 활성이 관찰되었으며, T. hatcheri의 에탄올추출물의 항균효과는 B. monroi의 에탄올추출물보다 강한 활성을 나타났다. 그렇지만 P388 murine leukaemia cells ($IC_{50}>12,500{\mu}g/mL$)에 대한 세포독성은 아주 미미한 것으로 나타났다.