Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Sequence Analysis of Nuclear 18S rDNA from Porphya tenera (Rhodophyta) in Korea

한국산 참김 (Porphya tenera)의 핵 18S rDNA염기서열 분석

  • JIN Long-Guo (Department of Biotechnology and Bioensineering, Pukyong National Univerisity) ;
  • KIM Myung-Sook (Department of Biotechnology and Bioensineering, Pukyong National Univerisity) ;
  • CHOI Jae-Suk (Department of Biotechnology and Bioensineering, Pukyong National Univerisity) ;
  • CHO Ji-Young (Department of Biotechnology and Bioensineering, Pukyong National Univerisity) ;
  • JIN Duck-Hee (Faculty of Marine Biotechnology, Kangnung National University) ;
  • HONG Yong-Ki (Department of Biotechnology and Bioensineering, Pukyong National Univerisity)
  • ;
  • 김명숙 (부경대학교 생물공학과) ;
  • 최재석 (부경대학교 생물공학과) ;
  • 조지영 (부경대학교 생물공학과) ;
  • 진덕희 (강릉대학교 해양생물공학부) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2003.02.01
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Abstract

Nuclear 18S ribosomal RNA gene (185 rDNA) from the aquaculturable seaweed Porphya tenera (Bangiales, Rhodophyta) was amplified using the polymerase chain reaction and its sequence was analysed. Complete 185 rDNA has an 1,822 bp exon and a 510 bp intron. The G+C contents of exon and intron were $48.68\%\;and\;54,90\%,$ respectively. The exon sequence showed $99.6\%$ homology to the GebBank accession number AB029880 of the Japanese P. tenera. The intron region that is inserted upstream between 568 and 1,079 showed $43.6\%$ homology to the AB029880.

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References

  1. Brodie, J., P.K. Hayes, G.L. Barker, L.M. Irvine and I. Bartsch. 1998. A reappraisal of Porphyra and Bangia (Bangiophycidae, Rhodophyta) in the northeast Atlantic based on the rbcL-rbcS intergenic spacer. J. Phycol., 34, 1069-1074 https://doi.org/10.1046/j.1529-8817.1998.341069.x
  2. Dutcher, J.A. and D.F. Kapraun. 1994. Random amplified polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales, Rhodophyta). J. Appl. Phycol., 6, 267-273 https://doi.org/10.1007/BF02181937
  3. Hendriks, L., R De Baere,Y. van De Peer, J. Neefs, A. Goris and R. Dewatcher. 1991. The evolutionary position of the Rhodophyte Porphyra umbilicalis and the Basidiomycete Leucosporidium scottii among other eukaryotes as deduced from complete sequences of small ribosomal subunit RNA. J. Mol. EvoI., 32, 167-177 https://doi.org/10.1007/BF02515389
  4. Hillis, D.M. and M.T. Dixon. 1991. Ribosomal DNA: Molecular evolution and phylogenetic inference. Quart. Rev. BioI., 66, 411-453 https://doi.org/10.1086/417338
  5. Hong, Y.K., S.D. Kim, M. Polne-Fuller and A. Gibor. 1995. DNA extraction conditions from Poyphyra perforata using LiCl. J. AppI. PhycoI., 7, 101-107 https://doi.org/10.1007/BF00693055
  6. Hwang, M.S., M.H. Han and I.K. Lee. 1998. Allozyme variation and species relationships in the genus Porphyra (Bangiales, Rhodophyta) from Korea. Algae, 13, 447-459
  7. Kunimoto, M., H. Kito, Y. Kaminishi, Y. Mizukami and N. Murase. 1999. Molecular divergence of the ssu rRNA gene and internal transcribed spacer 1 in Porphyra yezoensis (Rhodophyta). J. AppI. PhycoI., 11, 211-216 https://doi.org/10.1023/A:1008023525847
  8. Lee, I.K. and J.W. Kang. 1986. A check list of marine algae .in Korea. Kor. J. Phycol., 1, 311-325
  9. Ministry of Marine Affairs and Fisheries. 1997-2000. Statistical Yearbook of Marine Affairs and Fisheries. Republic of Korea, pp. 1-1132, Cheongwoo Moonwhasa, Seoul (In korean)
  10. Park, J.W., Y.C. Cho, B.H. Nam, H.J. Jin, C.H. Sohn and Y.K. Hong. 1998. RAPD identification of genetic variation in the seaweed Hizikie fusiformis. J. Mar. BiotechnoI., 6, 62-64
  11. Stiller, J.W. and J.R. Waaland. 1993. Molecular analysis reveals cryptic diversity in Porphyra (Rhodophyta). J. PhycoI., 29, 506-517 https://doi.org/10.1111/j.1529-8817.1993.tb00152.x
  12. White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. Innis, M., J. Gelfand, J. Sninsky and T. White eds., Academic Press, Florida, 315-322
  13. Yamazaki, S., Y. Kitade, T. Maruyama and N. Saga. 1996. Phylogenetic position of Porphyra yezoensis (Bangiales, Rhodophyta) based on the 18S rDNA sequence. J. Mar. BiotechnoI., 4, 230-232