Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Potential Species-Specific Marker in Several Fish Species by RAPD Using Universal Rice Primers

Universal Rice Primer (URP)-RAPD 방법에 의한 어류 종 특이 marker의 동정

  • KIM Woo-Jin (Biotechnology Research Center, National Fisheries Research and Development Institute) ;
  • KIM Kyung-Kil (Geoje Marine Hatchery, National Fisheries Research and Development Institute) ;
  • LEE Jeong-Ho (Biotechnology Research Center, National Fisheries Research and Development Institute) ;
  • PARK Doo-Won (Biotechnology Research Center, National Fisheries Research and Development Institute)
  • 김우진 (국립수산과학원 생명공학연구단) ;
  • 김경길 (국립수산과학원 거제수산시험장) ;
  • 이정호 (국립수산과학원 생명공학연구단) ;
  • 박두원 (국립수산과학원 생명공학연구단)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Morphologically similar fish species were subjected to the random amplified polymorphic DNA (RAPD) analysis using universal rice primer (URP). The fish species tested were sea basses (Lateolabrax japonicus and L. maculatus), eels (Anguilla japonica, A. bicolor bicolor, A. rostrata, and A. anguilla), and flounders (Limanda yokohamae and L. herzensteinin). Highly reproducible RAPD patterns were observed with several potential species-specific markers. The results indicate that RAPD technique using URP is useful for distinguishing fish psecies in a rapid manner.

Keywords

References

  1. Aoyama, J. and S. Watanabe. 2000. Discrimination of catadromous eels of genus AngUilla using polymerase 320 chain reaction-restriction fragment length polymorphism analysis of the mitochondria 16S ribosomal RNA domain. Trans. Am. Fish. Soc., 129, 873-878 https://doi.org/10.1577/1548-8659(2000)129<0873:DOCEOG>2.3.CO;2
  2. Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci., 62, 727-730 https://doi.org/10.2331/suisan.62.727
  3. Kang, E.J., K.S. Kim, S.R. Park and S.G. Sohn. 2000. Species identification of Japanese, American, and European eel elvers, and changes in morphometric characters according to growth. Kor. J. Ichthyol., 12, 244-249. (in Korean)
  4. Kang, H.W., Y.G. Cho and M.Y. Eun. 1997. DNA fingerprint of rice varieties (Oryza sativa L.) using primers designed from repetitive sequence of Korean red rice and its application to other organisms. 5th International Conference on Plant and Animal Genome. San Diego, CA, 81 pp
  5. Kim, I.S. and C.H. Youn. 1994. Taxonomic revision of the flounders (Pisces: Pleuronectiformes) from Korea. Kor. J. Ichthyol., 6, 99-131. (in Korean)
  6. Park, J.Y., K.K. Kim and Y. Kim. 1996. Genetic characterization of two types of sea bass, Lateolabrax japonicus in Korea by isozyme analysis. J. Aquacult., 9, 437-444. (in Korean)
  7. Patwary, M.D. E.L. Kenchington, C.J. Bird and E. Zouros. 1994. The use of random amplified polymorphic DNA markers in genetic studies of the sea scallop, Plscopecten magellanicus (Gmelin, 179 I). J. Shellfish Res., 13, 547-553
  8. Schoth, M. 1982. Taxonomic studies on the O-group eel larvae (Anguilla spec.) caught in the Sargasso Sea in 1979. Helgolander Meeresunters 35
  9. Takagi, M and N. Taniguchi. 1995. Random amplified polymorphic DNA (RAPD) for identification of three species of Anguilla: A. japonica, A. australis and A. bicolor. Fish. Sci., 61, 884-885 https://doi.org/10.2331/fishsci.61.884
  10. Tesch, F.W. 1999. Der Aal. Biologie und Fischerei. Verlag Paul Parey, Harnhurg
  11. Williams, J.G.K., A.R. Kugelik, K.J. Livak, J.A. Rafolski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res., 18, 6351-6535
  12. Williams, J.G.K, M.K. Hanafey, J.A. Rafolski and S.V. Tingey. 1993. Genetics analysis using random amplified polymorphic DNA markers. Methods Enzymol., 218, 704-740 https://doi.org/10.1016/0076-6879(93)18053-F