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Properties of Two Cellular Biomarker Parameters in the Blood of Farmed Pacific Oyster, Crassostrea gigas, Exposed to Polychlorinated Biphenyls

  • Choy Eun Jung (Aquaculture Department, National Fisheries Research and Development Institue) ;
  • Jo Qtae (Aquaculture Department, National Fisheries Research and Development Institue) ;
  • Do Jeong Wan (Pathology Department, National Fisheries Research and Development Institue) ;
  • Kim Sang Soo (Marine Environment Management Department, National Fisheries Research and Development Institute) ;
  • Jee Young-Ju (Aquaculture Department, National Fisheries Research and Development Institue) ;
  • Min Kwang Sik (Aquaculture Department, National Fisheries Research and Development Institue)
  • Published : 2003.06.01

Abstract

Two cellular biomarker parameters of the farmed Pacific oyster Crassostrea gigas were studied in vivo and in vitro after exposure to concentrations of polychlorinated biphenyls in terms of neural red uptake (NRU) and lysozyme activity. The oysters exposed in vivo to the xenobiotic concentrations, 0, 30, 90, and 180 ng/g for 14 days, enhanced hemocyte NRU with occasional significant differences (P<0.05), depending on the chemical concentration and duration. An adverse tendency was manifest in the lysozyme activities both in the hemocyte and serum of the oyster treated with the chemical in a same manner, rendering these two cellular parameters as biomarker candidates against the chemical. The oysters exposed in vitro to the chemical concentrations, 0, 1, 5, 10, 100, 1,000, and 10,000 ng/g for 24 hrs at $10^{\circ}C$ showed a similar tendancy as those exposed in vivo to the chemical. Unlike in vivo response, however, the in vitro NRU was first influenced by very low concentration of the chemical. In in vitro results, marked but not significant increase of hemocyte NRU was noticed at the chemical concentration of 5 ng/g, where the value was almost as high as those exposed to higher chemical concentrations, up to 10,000 ng/g. An unusual result was observed in the in vitro lysozyme activity of hemocyte in which significant decrease was first noticed at the chemical concentration of 100 ng/g.

Keywords

References

  1. Allam, B. and C. Paillard. 1998. Defense factors in clam extrapallial fluids. Dis. Aquat. Organ., 33, 123-128 https://doi.org/10.3354/dao033123
  2. Anderson, R.S. 1996. Interaction of Perkinsus marinus with himoral factors and hemocytes of Crassostrea virginica. J. Shellfish Res., 5, 127-134
  3. Anderson, R.S., L.L. Brubacher, L.M.R. Calvo, E.M. Burreson and M.A. Unger. 1997. Effect of in vitro exposure to tributyltin on generation of oxygen metabo ites by oyster hemocytes. Environ. Res., 74, 84-90 https://doi.org/10.1006/enrs.1997.3751
  4. Carballal, M., C. Lopez, C. Azevedo and A. Villalba. 1997. Enzymes involved in defence functions of hemocytes of mussel Mytilus galloprovincialis. J. Invertebr, Pathol., 70, 96-105 https://doi.org/10.1006/jipa.1997.4670
  5. Cajaraville, M.P., I. Olabarrieta and I. Marigomez. 1996. In vitro activities in mussel hemocytes as biomarkers of environmental quality: a case study in the Abra estuary (Biscay Bay). Ecotoxicol. Environ. Saf., 35, 253-260 https://doi.org/10.1006/eesa.1996.0108
  6. Cheng, T.C. and G.E. Rodrick. 1974. Identification and characterization of lysozyme from the hemolymph of the softshclled clam, Mya arenaria. BioI. Bull.,147, 311-320 https://doi.org/10.2307/1540451
  7. Cheng, T.C., G.E. Rodrick, D.A. Foley and S.A. Koehler. 1975. Release of lysozyme from the hemolymph cells of Mer cenaria mercenaria during phagocytosis. J. Invertebr. Pathol., 27, 125-128 https://doi.org/10.1016/0022-2011(76)90036-7
  8. Cheng, T.C.. 1981. Bivalves. In: Invertebrate Blood Cells Vol. 1, N.A. Ratcliffe, A.F. Rowely. eds., Academic Press, London, pp. 233-300
  9. Cheng, T.C., 1983. The role of Iysosomes in molluscan inflamation. Am. Zool., 23, 129-144 https://doi.org/10.1093/icb/23.1.129
  10. Chu, F.L.E. and J.F. La Peyre. 1989. Effect of environmental factor and parasitism on hemolymph lysozyme and protein of American oyster (C. virginica). J. Invertebr. Pathol., 54, 224-232 https://doi.org/10.1016/0022-2011(89)90032-3
  11. Chu, F.L.E., A.K Volety, R.e. Hale and Y. Huang. 2002. Cellular responses and disease expression in oysters (Crassostrea virginica) exposed to suspended field-contaminated sediments. Mar. Environ. Res., 53, 17-35 https://doi.org/10.1016/S0141-1136(01)00104-0
  12. Coles, J.A., R. Farley and R.K. Pipe. 1995. Effects of fluoranthene on the immunocompetence of the common marine mussel Mytilus edulis. Aquat. Toxicol., 30, 367-379 https://doi.org/10.1016/0166-445X(94)00051-4
  13. Cronin, M.A., S.C. Culloty and M.F. Mulcahy. 2001. Lysozyme activity and protein concentration in the haemolymph of the flat oyster Ostrea edulis (L.). Fish Shellfish Immunol., 11, 611-622 https://doi.org/10.1006/fsim.2001.0338
  14. Etxeberria, M., I. Sastre., M.P. Cajaraville and I. Marigomez. 1994. Digestive lysosome enlargement induces by experimental exposure to metals (Cu, Cd, and Zn) in mussels collected from a zinc-polluted site. Arch. Environ. Contam. Toxicol., 24, 338-345
  15. Fagotti, A., I. Di Rosa. F. Simoncelli. R.K. Pipe. F. Panaraand and R. Pascolini. 1996. The effects of copper on the actin and fibronectin in Mytilus galloprovincialis hemocytes. Dev. Compo Immunol., 20, 383-392 https://doi.org/10.1016/S0145-305X(96)00021-3
  16. Faisal, M. and S. Demmerle-Sami. 1994. Polyaromatic hydrocarbons modulate the macromolecular synthesis in haemocytes of the Eastern oyster (Crassostrea virginica). In: Modulators of Fish Immune Responses, Vol. 1. J.S. Stolen. T.C. Fletcher. eds., SOS Publications, Fair Haven, pp. 235-246
  17. Goldberg, E.D. 1986. The mussel watch concept. Environ. Monit. Assess., 7, 91-103 https://doi.org/10.1007/BF00398031
  18. Grundy, M.M., M.N. Moore., S.M. Howell and N.A. Ratcliffe. 1996a. Phagocytic reduction and effects on lysosomal membranes by polycyclic hydrocarbons, in haemocytes of Mytilus edulis. Aquat. Toxicol., 34, 273-290 https://doi.org/10.1016/0166-445X(95)00044-5
  19. Grundy. M.M., N.A. Ratcliffe and M.N. Moore. 1996b. Immune inhibition in marine mussels by polyclclic aromatic hydrocarbons. Mar. Environ. Res., 42, 187-190 https://doi.org/10.1016/0141-1136(95)00033-X
  20. Hutchinson, T.H. and M.J. Manning. 1996. Seasonal trends in serume lysozyme activity and total protein concentration in dab (Limanda limanda L.) sampled from Lyme Bay, UK. Fish Shellfish Immunol., 6, 473-482 https://doi.org/10.1006/fsim.1996.0045
  21. Jo, Q., E.J. Choy, D.W. Park, Y.J. Jee, S.Y. Kim and Y. Kim. 2002. Cellular biomarker of membrane stability and hydrolytic enzyme activity in the hemocytes of benzo(a)pyrene-exposed Pacific oyster, Crassostrea gigas. J. Fish. Sci. Tech., 5, 263-270
  22. Kang, C.K., M.S. Park, P.Y. Lee, W.J. Choi and W.C. Lee. Seasonal variations in condition, reproductive activity, and biochemical composition of the Pacific oyster, Crassostrea gigas (Thunberg), in suspended culture in two coastal bays of Korea. J. Shellfish Res., 19, 771-778
  23. Larson, K.G., B.S. Robertsom and F.M. Hetrick. 1989. Effect of environmental pollutants on the chemoluminescence of hemocytes from the American oyster, Crassostrea virginica. Dis. Aqat. Org., 6, 203-210
  24. Livingstone, D.R., P. Donkin and C.H. Walker. 1992. Pollutants in marine ecosystems: an overview. In: Persistent Pollutants in Marine Ecosystems. C.H. Walker, D.R. Livingstone. eds., Pergamon Press, Oxford, pp. 235-263
  25. Livingstone, D.R., J.K. Chipman, M.D. Lowe, C. Minier, C.L, Mitchelmore, M.N. Moore, L.D. Peters and R.K. Pipe. 2000. Development of biomarkers to detect the effects of organic pollution on aquatic invertebrates: recent molecular, genotoxic, cellular and immunological studies on the common mussel (Mytilus edulis L.) and other mytilids. Int. J. Environ. Pollut., 13, 56-91 https://doi.org/10.1504/IJEP.2000.002311
  26. Lowe, D.M., M.N. Moore and B.M. Evans. 1992. Contaminant impact in interactions of molecular probes with lysosomes in living hepatocytes from dab Limanda limanda, Mar. Ecol. Prog. Ser., 91, 135-140 https://doi.org/10.3354/meps091135
  27. Lowe, D.M. and R.K. Pipe. 1994. Contaminants induced lysosomal membrane damage on marine mussel digestive cells: an in vitro study. Aquat. Toxicol., 30, 357-365 https://doi.org/10.1016/0166-445X(94)00045-X
  28. Matozzo, V., L. Ballarin and M.G. Marin. 2002. In vitro effects of tributyltin on functional responses of haemocytes in the clam, Tapes philippinarum. Appl. Organometal. Chem.,16, 169-174 https://doi.org/10.1002/aoc.278
  29. McDade, J.E. and M.R. Tripp. 1967a. Lysozyme in oyster mantle muscus. J. Invertebr. Pathol., 9, 581-582 https://doi.org/10.1016/0022-2011(67)90146-2
  30. MaDade, J.E. and M.R. Tripp. 1967b. Lysozyme in the hemolymph of the oyster C. virginica. J. Invertebr. Pathol., 9, 531-535 https://doi.org/10.1016/0022-2011(67)90134-6
  31. Moore, M.N. 1990. Lysosomal cytochemistry in marine environmental monitoring. Histochem. J., 22, 189-191
  32. NFRDI Report. 1997. Studies on the bad seed collection in oyster growing area and investigation of the new oyster seed collection area. National Fisheries Research and Development Institute, Pusan, Korea, pp. 1-226
  33. Pickwell, G.V. and S.A. Strinert. 1984. Serume biochemical and cellular responses to experimentally cupric ion challenge in mussels. Mar. Environ. Res., 14, 245-265 https://doi.org/10.1016/0141-1136(84)90081-3
  34. Pipe, R.K., J.A. Coles and S.R. Farley. 1995. Assays for measuring immune response in the mussel Mytilus edulis. Tech. Fish Immunol., 4, 93-100
  35. Rodrick, G.E. and T.C. Cheng. 1974. Kinetic properties of lysozyme from the hemolymph of Crassostrea virginica. J. Invertbr. Pathol., 24, 41-48 https://doi.org/10.1016/0022-2011(74)90162-1
  36. Widdows, J. and P. Donkin. 1992. Mussels and environmental contaminants: bioaccumulation and physiological aspects. In: The Mussel Mytilus: Ecology, Physiology, Genetics and Aquaculture. E. Gosling. ed., Elsevier, Amsterdam, pp. 383-424

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