Journal of Yeungnam Medical Science

Yeungnam University College of Medicine, Yeungnam University Institute Medical Science (영남대 의대 학술지 편집위원회)
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Bone Formation by rhBMP-7 Transduced HEK 293 Cells in Nude Mouse

재조합 BMP-7 유전자가 전달된 HEK 293 세포에 의한 누드 마우스에서의 뼈형성

  • Jeong, Su-Yon (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Chang, Won-Tae (Department of Anatomy, College of Medicine, Yeungnam University) ;
  • Chang, Yon-Sil (Department of Biochemistry.Molecular biology, College of Medicine, Yeungnam University) ;
  • Ahn, Myun-Hwan (Department of Orthopedic surgery, College of Medicine, Yeungnam University) ;
  • Kim, Jae-Ryong (Department of Biochemistry.Molecular biology, College of Medicine, Yeungnam University) ;
  • Song, In-Hwan (Department of Anatomy, College of Medicine, Yeungnam University)
  • 정수연 (영남대학교 의과대학 해부학교실) ;
  • 장원태 (영남대학교 의과대학 해부학교실) ;
  • 장연실 (영남대학교 의과대학 생화학.분자생물학교실) ;
  • 안면환 (영남대학교 의과대학 정형외과학교실) ;
  • 김재룡 (영남대학교 의과대학 생화학.분자생물학교실) ;
  • 송인환 (영남대학교 의과대학 해부학교실)
  • Published : 2003.12.30
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Abstract

To induce bone formation at ectopic site by tissue engineering and gene therapy, we transplanted collagen sponges containing rhBMP-7 transduced HEK 293 cells in the hypodermis of nude mice. Bone formation was investigated by histological and electron microscopic method at 3, 6, and 9 weeks after transplantation. At 9 weeks after transplantation, eosinophilic bony tissue was observed in the implanted collagen sponge and was confirmed as bone tissue by Von Kossa stain. In the transmission electron microscopic observation, the cells in newly formed bone tissue had eccentrically located nucleus and well developed rough endoplasmic reticulum (rER). Therefore, the cells were evaluated as osteoblasts. Those results suggest that it is possible to form a bone tissue in the ectopic site by transplantation of rhBMP-7 transduced HEK 293 cells. This will be contributed to push more advanced gene therapy for bone formation. However, the HEK 293 cell is unable to apply to the clinical gene therapy. Therefore it is worth to find more compatible cells for clinical application. In addition, collagen sponge is considered as an excellent scaffold and/or carrier for gene therapy and a good biomaterial for tissue engineering.

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