인삼이 사람태아골모세포의 세포주기조절에 미치는 영향

Effects of Ginseng Radix on the Cell Cycle Regulation in Human Fetal Osteoblast

  • 김대겸 (원광대학교 치과대학 치주과학교실) ;
  • 이용배 (원광대학교 치과대학 치주과학교실) ;
  • 박상기 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 유경태 (원광대학교 치과대학 치주과학교실) ;
  • 김윤철 (원광대학교 약학대학 약학과) ;
  • 신형식 (원광대학교 치과대학 치주과학교실)
  • Kim, Dae-Gyeom (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lee, Yong-Bae (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Park, Sang-Kee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Kyung-Tae (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Yun-Chul (Department of Pharmacy, Pharmacy College, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 발행 : 2003.09.30

초록

Ginseng Radix(GR) had been used widely from oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The results were as follows. Increased cell proliferation was observed in cells exposed to 100 ng/ml, 10 ng/ml of GR-1 at 12 hours and 24 hours, 1 ${\mu}g$/ml of GR-1 at 48 hours, and 100 ${\mu}g$/ml, 10 ${\mu}g$/ml of GK-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2. CDK 4 and CDK 6 were increased in the group of treated with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3. On the other hand, p21 was decreased in the treatment group with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, 10 ${\mu}g$/ml of GR-3, and p53 and p16 was decreased in the treatment group with 100 ng/ml of GR-1, 100 ${\mu}g$/ml and GR-3 and pRb was decreased in the all treatment groups except 1 ${\mu}g$/ml of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1 , Cyclin E, CDK 2, CDK 4, CDK 6 and decreased cell cycle regulation protein levels of p21, pRb.

키워드

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