Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Isolation of Fusobacterium nucleatum from subgingival plaque in Korean

한국인의 치은연하 치태에서 Fusobacterium nucleatum의 분리

  • Jang, Hyun-Seon (Department of Periodontology, Chosun University, Oral Biology Research Institute, College of Dentistry) ;
  • Kim, Seo-Hoon (Department of Periodontology, Chosun University) ;
  • Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Oral Biology Research Institute) ;
  • Kim, Mi-Kwang ;
  • Yoo, So-Young ;
  • Kim, Byung-Ock (Department of Periodontology, Chosun University, Oral Biology Research Institute, College of Dentistry)
  • 장현선 (조선대학교 치과대학 치주과학교실, 구강생물학연구소) ;
  • 김세훈 (조선대학교 치과대학 치주과학교실) ;
  • 김화숙 (구강생화학교실) ;
  • 국중기 (구강생화학교실, 구강생물학연구소) ;
  • 김미광 (Department of Oral Biochemistry, College of Dentistry) ;
  • 유소영 (Department of Oral Biochemistry, College of Dentistry) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 구강생물학연구소)
  • Published : 2003.06.30
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Abstract

The purpose of this study was to isolate and characterize the Fusohacrerium nucleatum (F. nucleatum) from subgingival plaque in Korean periodontitis patients. The subgingival plaque samples of periodontitis patient were collected with sterilized paper point. The paper point was put into reduced transfer medium and then immediately transferred to laboratory. The subgingival samples were diluted by 10,000 folds and plated on F. nucleatum-selective media agar plate. The plates were incubated at 37$^{\circ}C$ in an anaerobic chamber for 3 days. The violet-colored colonies were selected and subjected to further verification whether those are F. nucleatum or not. For further confirmation, 16S rRNA genes (rDNA) were cloned from each of bacterial clones and determined sequence of 16S rDNA. In this study, we found 17 distinct clinical isolates of F. nucleatum from subgingival plaque. The clinical isolates will be a useful in various studies in periodontology.

Keywords

References

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