Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of sonicates of Treponema denticola on osteoblast differentiation

Treponema denticola 분쇄액에 의한 조골세포분화 억제효과

  • Choi, Bong-Kyu (Department of Oral Biology, Yonsei University, Brain Korea 21 Project for Medical Sciences, Yonsei University) ;
  • Kang, Jung-Hwa (Department of Oral Biology, Yonsei University) ;
  • Jin, Seung-Wook (Department of Oral Biology, Yonsei University) ;
  • Ohk, Seung-Ho (Department of Oral Biology, Yonsei University, Brain Korea 21 Project for Medical Sciences, Yonsei University) ;
  • Lee, Syung-Il (Department of Oral Biology, Yonsei University, Oral Science Research Center, College of Dentistry, Brain Korea 21 Project for Medical Sciences, Yonsei University) ;
  • Yoo, Yun-Jung (Department of Oral Biology, Yonsei University, Oral Science Research Center, College of Dentistry)
  • 최봉규 (연세대학교 치과대학 구강생물학교실, 연세대학교 의과대학 BK 의과학사업단) ;
  • 강정화 (연세대학교 치과대학 구강생물학교실) ;
  • 진승욱 (연세대학교 치과대학 구강생물학교실) ;
  • 옥승호 (연세대학교 치과대학 구강생물학교실, 연세대학교 의과대학 BK 의과학사업단) ;
  • 이승일 (연세대학교 치과대학 구강생물학교실, 연세대학교 치과대학 구강과학연구소, 연세대학교 의과대학 BK 의과학사업단) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실, 연세대학교 치과대학 구강과학연구소)
  • Published : 2003.03.30
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Abstract

치주질환은 세균감염에 의해 치조골이 파괴되는 염증성질환으로서 치아상실의 주된 원인이다. Treponema denticola는 성인성 치주염의 병소에서 자주 발견되는 세균으로서 부착능 및 단백분해효소생성능과 같은 독성 인자가 밝혀져 치주조직 파괴에 있어서 중요성이 강조되어 왔다. 골개조는 조골세포의 골형성및 파골세포에 의한 골흡수의 균형에 의하여 유지되며 치주염시 야기되는 치조골파괴는 조골세포 및 파골세포 기능의 불균형에 의하여 야기되는 것으로 설명되고 있다. 골세포에 대한 영향으로서 T. denticola는 파골세포의 형성을 촉진시키는 것으로 보고되었으나 조골세포에 대한 영향은 아직 밝혀져 있지 않다. 따라서 본 연구에서는 T. denticola가 골형성에 미치는 영향을 알아보고자 마우스의 두개골세포로부터 조골세포를 분리한 후 T. denticola분쇄액으로 처리하여 본 세균이 조골세포의 alkaline phosphatase(ALPase) 활성, 석회화결절 형성 및 Prostaglandin $E_2\;(PGE_2)$ 생성에 미치는 영향을 평가하였다. ALPase활성은 p-nitrophenylphosphate분해능, 석회화결절형성은 Von Kossa 염색법, 그리고 PGE2의 농도는 효소면역측정법으로 측정하였다. T. denticola분쇄액 (2.5 ug/ml)은 마우스 두개골세포의 ALPase활성을 억제하였으며 석회화결절의 형성을 감소시켰다. 또한 동일한 농도의 균분쇄액은 마우스 두개골세포의 $PGE_2$ 생산을 증가시켰다. 균분쇄액과 prostaglandin의 합성억제제인 indomethacin으로 세포를 동시에 처리한 경우 T .denticola분쇄액에 의한 $PGE_2$의 생산은 감소되었으나, ALPase의 활성억제에는 변화가 없었다. 균분쇄액을 열처리하여 마우스 두개골세포에 처리하였을 때에도 ALPase의 활성이 억제되는 것에는 변함이 없었다. 이러한 결과는 T. denticola의 구성성분 중 열에 안정한 물질이 prostaglandin과 무관한 경로를 통해 조골세포의 분화를 억제함을 시사하며 이와 같은 T. denticola에 의한 골형성억제가 치주염시 야기되는 치조골 파괴에 관여할 수 있을 것으로 생각된다.

Keywords

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