Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Biological Activities of HA-coated Zirconia

HA-coated Zirconia의 생물학적 활성도에 관한 연구

  • Nam, Suk-Woo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Kim, Hae-Won (School of Materials Science and Engineering, Seoul National University) ;
  • Kim, Hyoun-Ee (School of Materials Science and Engineering, Seoul National University) ;
  • Yang, Seung-Min (Department of Periodontology, College of Dentistry, Seoul National University, Department of Dentistry, College of Medicine, Sungkyunkwan University) ;
  • Shin, Seung-Youn (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Choi, Sang-Mook (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, College of Dentistry, Seoul National University)
  • 남석우 (서울대학교 치과대학 치주과학교실) ;
  • 김해원 (서울대학교 공과대학 료공학부) ;
  • 김현이 (서울대학교 공과대학 료공학부) ;
  • 양승민 (서울대학교 치과대학 치주과학교실, 성균관대학교 의과대학 치과학교실) ;
  • 신승윤 (서울대학교 치과대학 치주과학교실) ;
  • 이용무 (서울대학교 치과대학 치주과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 최상묵 (서울대학교 치과대학 치주과학교실) ;
  • 류인철 (서울대학교 치과대학 치주과학교실)
  • Published : 2003.03.30
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Abstract

Hydroxyapatite(HA) has been extensively used as bone graft materials and tooth implant surface coating materials because of its biocompatibility and osteoconductive properties. However, as HA is intrinsically poor in mechanical properties, zirconia($ZrO_2$) was incorporated with HA as reinforcing phases for improvement of mechanical properties. The purpose of this study was to investigate the biological activities of HA-coated zirconia through the cell proliferation test, measurements of alkaline phosphatase activity, and histologic examination. Four kinds of tested blocks were prepared according to the pore size (300-500${\mu}m$/500-700${\mu}m$) and the porosity (70%/90%). Cell proliferation and alkaline phosphatase activity was measured at 1, 7, 14 days. The number of cells proliferate after 7, 14 days were significantly increased in all groups when compared with that of the first day, but there was no significant difference between the 4 groups at each time period. At the 7 day, alkaline phosphatase activities of cells cultured in 4 groups were higher than that of the first day, but there was no significant difference between the 4 groups at each time period. The human gingival fibroblast and MG 63 cell was used to evaluate the cell cytotoxicity using MTT test. The materials tested in the current study turned out to be non-cytotoxic. In histologic examination(SEM), at 1 day there were many cells attached on the surfaces of all kinds of tested blocks. The number of cells were increased over time. At the 14 day, there were more cells proliferated than 1 day and some of the pores of blocks were partially filled with the proliferated cells. The in vitro response of osteoblast-like cells to the HA-coated zirconia showed comparable effect on transformation comparable to hydroxyapatite.

Keywords

References

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