분석과학 (Analytical Science and Technology)

  • 제16권3호
  • /
  • Pages.179-184
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  • 2003
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  • 1225-0163(pISSN)
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  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
권호 표지

유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제

Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. var. japonica Nakai

  • 한기정 (중앙대학교 자연과학대학 화학과) ;
  • 이광수 (중앙대학교 자연과학대학 화학과) ;
  • 공광훈 (중앙대학교 자연과학대학 화학과) ;
  • 조성희 (중앙대학교 자연과학대학 화학과)
  • 투고 : 2002.06.17
  • 심사 : 2003.05.27
  • 발행 : 2003.06.25
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초록

극성이 다른 여러 가지 용매를 사용하여 느릅나무의 뿌리 껍질(이하 유근피)을 추출하였다. MeOH 추출물이 SK-Hep-1에서 분리된 matrix metalloproteinase-9(MMP-9)의 zymography에서 저해능을 나타내었다. 분리 정제한 물질의 분자량은 GC-MS spectrum에서 $M^+=281$인 것으로 나타났고 MMP-9의 활성은 $314.7{\mu}g/g$에서 47% 억제되는 것으로 나타났다. 그리고 SK-Hep-1 세포주는 $31.47{\mu}g/g$에서 60%의 생존율을 나타났다.

Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of $314.7{\mu}g/g$. Cell viability of SK-Hep-1 was 60% at $31.47{\mu}g/g$.

키워드

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