Journal of the Korean Wood Science and Technology
- 제31권1호
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- Pages.16-21
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- 2003
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- 1017-0715(pISSN)
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- 2233-7180(eISSN)
느릅나무 근피의 화학조성분 및 항균성 물질(II) : 항균성 물질의 단리 및 화학구조
Chemical Analysis and Isolation of Antibacterial Compound from Ulmus Species (II) : Isolation and Chemical Structure of Antibacterial Compound
- Kim, Chang-Soon (Kyungpook National University) ;
- Lee, Jung-Myoung (Kyungpook National University) ;
- Choi, Chang-Ok (Kyungpook Forest Environmental Institute) ;
- Park, Soung-Bae (Kyungpook National University) ;
- Eom, Tae-Jin (Kyungpook National University)
- 투고 : 2002.03.13
- 심사 : 2002.05.30
- 발행 : 2003.02.26
초록
느릅나무 근피의 메탄올 추출물을 분획하여 항균성을 측정한 결과 항균력이 가장 우수한 수용성 분획을 silicagel column chromatograpy 및 TLC에 의해 분리·정제한 다음 정제 화합물을 UV, IR, 1H-NMR, 13C-NMR를 사용하여 구조를 동정하고 그 화합물의 항균력을 검정하였다. 수용성 분획물로부터 두 종류의 정제 화합물을 단리하였다. 기기분석 결과, 화합물 B는 Catechin rhamnoside로 확인되었다. 단리된 정제화합물은 Staphylococcus aureus 및 Salmonella typhimurium에 대하여 항균활성을 나타내었다.
The methanol extractives from root bark of Ulmus davidiana var japonica nakai were fractionated with n-hexane, ethyl ether, ethyl acetate and waster, the water soluble fraction was also fractionated with silicagel column chromatograhy. The chemical structure of purifided compounds were identified with UV, IR, 1H-NMR and 13C-NMR spectra and the antibacterial activities also were investigated. Two different antibacterial compounds (compound A and B) were fractionated with silicagel chromatography and TLC. Compounds B was identified as a catechin rahmnoside. The both of compounds had antibacterial activity on Staphylococcus aureus and Salmonella typhimurium.
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