Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Sophorae Radix Extract in Rat Cardiac Endothelial Cells

고삼 추출물이 배양 심장내피세포에 미치는 영향

  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Park Cheon Su (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Gyu (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Hyun Gyu (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Choi Ki Bang (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Yong Bok (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 박천수 (원광대학교 한의과대학 생리학교실) ;
  • 김인규 (원광대학교 한의과대학 생리학교실) ;
  • 김현규 (원광대학교 한의과대학 생리학교실) ;
  • 최기방 (원광대학교 한의과대학 생리학교실) ;
  • 김용복 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2003.02.01
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Abstract

To test the protective effect of Sophorae Radix (SR) on the damage of cardiac endothelial cells by xanthine oxidase (XO)/hypoxanthine (HX)-induced oxygen tree radical, Neutral Red (NR), lactate dyhydrogenase (LDH), and c-fos immunopositive cells assay were used in the presence of SR extract. The results of these experiments were obtained as follows ; Cardiac endothelial cells treated with XO/HX showed the cytotoxicity such as a decrease in viability, and increases in LDH activity and c-fos immunopositive cells. Cardiac endothelial cells pretreated with SR extract protected the increase of LDH activity. Alos, cardiac endothelial cells pretreated with SR extract inhibited the increase of c-fos immunopositive cells. These results show that XO/HX induces toxic effects in cultured cardiac endothelial cells derived from neonatal rat, and suggest that SR extract is very effective in the prevention of XO/HX-induced toxicity.

Keywords

References

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